Fig. 2

DF rhythms in Brassica change with the age of the plant and between different leaf ages. Brassica seedlings were grown to 4 different ages: 20, 25, 30 and 35 days after sowing. Leaves 1, 3 and 5 were sampled from each plant in the experiment with leaf 1 being the earliest emerged leaf and leaf 5 the most recently emerged leaf. Boxplots show differences in period (a) and RAE (b) for each leaf age within each plant age. Colour scales reflect an ageing gradient with lighter colours representing younger material. Periods and RAE estimates were calculated using FFT NLLS (BAMP dtr, 24–120 h cut-off). Data represents results from two imaging cabinets run in parallel as technical replicates and normalised for the between-cabinet effects. Age 20: leaf 1 (N = 6/6), leaf 3 (N = 6/6), leaf 5 (N = 6/6). Age 25: leaf 1 (N = 4/6), leaf 3 (N = 6/6), leaf 5 (N = 6/6). Age 30: leaf 1 (N = 2/6), leaf 3 (N = 5/6), leaf 5 (N = 6/6). Age 35: leaf 1 (N = 6/6), leaf 3 (N = 6/6), leaf 5 (N = 6/6). Significance codes: ***p < 0.001, **p < 0.01 *p < 0.05, all significance markers are relative to leaf 1 at each age