Table 2 Primers for indexing PCR (indexed sequencing primers)
From: A high-throughput amplicon-based method for estimating outcrossing rates
Primer name | Sequence | Index set origin | index name |
|---|---|---|---|
F1_MetaIndex | AATGATACGGCGACCACCGAGATCTACACTATAGCCTTCGTCGGCAGCGTC | TruSeq i5 | D501 |
F2_MetaIndex | AATGATACGGCGACCACCGAGATCTACACATAGAGGCTCGTCGGCAGCGTC | TruSeq i5 | D502 |
F3_MetaIndex | AATGATACGGCGACCACCGAGATCTACACCCTATCCTTCGTCGGCAGCGTC | TruSeq i5 | D503 |
F4_MetaIndex | AATGATACGGCGACCACCGAGATCTACACGGCTCTGATCGTCGGCAGCGTC | TruSeq i5 | D504 |
F5_MetaIndex | AATGATACGGCGACCACCGAGATCTACACAGGCGAAGTCGTCGGCAGCGTC | TruSeq i5 | D505 |
F6_MetaIndex | AATGATACGGCGACCACCGAGATCTACACTAATCTTATCGTCGGCAGCGTC | TruSeq i5 | D506 |
F7_MetaIndex | AATGATACGGCGACCACCGAGATCTACACCAGGACGTTCGTCGGCAGCGTC | TruSeq i5 | D507 |
F8_MetaIndex | AATGATACGGCGACCACCGAGATCTACACGTACTGACTCGTCGGCAGCGTC | TruSeq i5 | D508 |
R13_MetaIndex | CAAGCAGAAGACGGCATACGAGATGTCGTGATGTCTCGTGGGCTCGG | TruSeq Amplicon | A701 |
R14_MetaIndex | CAAGCAGAAGACGGCATACGAGATCGAGTAATGTCTCGTGGGCTCGG | TruSeq i7 | D701 |
R15_MetaIndex | CAAGCAGAAGACGGCATACGAGATTCTCCGGAGTCTCGTGGGCTCGG | TruSeq i7 | D702 |
R16_MetaIndex | CAAGCAGAAGACGGCATACGAGATAATGAGCGGTCTCGTGGGCTCGG | TruSeq i7 | D703 |
R17_MetaIndex | CAAGCAGAAGACGGCATACGAGATGGAATCTCGTCTCGTGGGCTCGG | TruSeq i7 | D704 |