Table 1 Primers for Primary PCR
From: A high-throughput amplicon-based method for estimating outcrossing rates
Primer name | Sequence |
|---|---|
1_Carubv10013869m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCACTCATCCATTCGGAAAT |
1_Carubv10013869m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTTGGGGACAAGGTGCTAATC |
2_Carubv10023806m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTACCGACCACATAGGCATCA |
2_Carubv10023806m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAATGGCCGATTCTGCTTTTA |
3_Carubv10018138m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCAAGCCAAAGTTTGATGCTT |
3_Carubv10018138m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACTCGTCTGCAGTCATGGTG |
4_Carubv10001640m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGCGGATGGTTACAAAA |
4_Carubv10001640m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAGGCCAAGCTCACTCACATT |
5_Carubv10001924m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGGGTTCAGATTGAGCGTAA |
5_Carubv10001924m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAACTTGATCCTCTTTGGTACTGG |
6_Carubv10023818m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTTCTTTTTCTGAGATTCCATTGCT |
6_Carubv10023818m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAGAAGCCTCTCCTGAGAAGTGA |
7_Carubv10005658m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCCAAGATCTGTGCTTGCTG |
7_Carubv10005658m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTCAGCTCCGGATGGTTAAAT |
8_Carubv10006001Â m _F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTTTCAAAAGCTTTGCGTGAG |
8_Carubv10006001Â m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGATGCTTCACGTTCACACCA |
9_Carubv10006101m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTTCTATCCAAGGGCCATCA |
9_Carubv10006101m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCCATGGAAACTCCTTGTTG |
10_Carubv10027375m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGATCCGTCGGCTCTTCTCTC |
10_Carubv10027375m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAACCATGCCAATGCTTCATA |
11_Carubv10011729m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAGCAAGTCCCAAACAAAG |
11_Carubv10011729m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCATTTCAAGCCGCTCTGG |
12_Carubv10014733m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGCATTCGATCTCGATCTTG |
12_Carubv10014733m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGGTGGTGAAGACAACAATC |
Primers targeting same sequences as above, but with added T or A before gene-specific sequence for heterogeneity | |
1A_Carubv10013869m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACCACTCATCCATTCGGAAAT |
1A_Carubv10013869m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGATTGGGGACAAGGTGCTAATC |
2T_Carubv10023806m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTTACCGACCACATAGGCATCA |
2T_Carubv10023806m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTAATGGCCGATTCTGCTTTTA |
3A_Carubv10018138m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACAAGCCAAAGTTTGATGCTT |
3A_Carubv10018138m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAACTCGTCTGCAGTCATGGTG |
4T_Carubv10001640m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGGAAGCGGATGGTTACAAAA |
4T_Carubv10001640m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTAGGCCAAGCTCACTCACATT |
5A_Carubv10001924m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGGGTTCAGATTGAGCGTAA |
5A_Carubv10001924m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAAACTTGATCCTCTTTGGTACTGG |
6T_Carubv10023818m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTTTCTTTTTCTGAGATTCCATTGCT |
6T_Carubv10023818m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTAGAAGCCTCTCCTGAGAAGTGA |
7A_Carubv10005658m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATCCAAGATCTGTGCTTGCTG |
7A_Carubv10005658m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGATCAGCTCCGGATGGTTAAAT |
8T_Carubv10006001m _F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTTTTCAAAAGCTTTGCGTGAG |
8T_Carubv10006001m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTGATGCTTCACGTTCACACCA |
9A_Carubv10006101m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGTTCTATCCAAGGGCCATCA |
9A_Carubv10006101m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACCCATGGAAACTCCTTGTTG |
11T_Carubv10011729m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGGAGCAAGTCCCAAACAAAG |
11T_Carubv10011729m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTCATTTCAAGCCGCTCTGG |
12A_Carubv10014733m_F | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCATTCGATCTCGATCTTG |
12A_Carubv10014733m_R | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACGGTGGTGAAGACAACAATC |