Fig. 2
From: A high-throughput amplicon-based method for estimating outcrossing rates
Primer-test for individual primers and pooling strategy. a Primer sets 1–12 were tested with genomic DNA as template (odd-numbered lanes, ‘ + ’) or in a no-template control reaction (even-numbered lanes, ‘ − ’). Primer set 10 (lanes 19 and 20) gave no PCR product and was therefore excluded in further steps. b Pooled amplification of primer sets 1–5 (lanes 1 and 2) and 6–12 (without 10, lane 3 and 4) with water controls