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Table 2 Quantitation of ubiquitin and BSA standards as determined by LC–MS/MS isotope dilution and AccQ-Tag methods

From: Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry

 

H2O2 + 6 M HCl + β-ME

6 M HCl + β-ME

4 M MetS + 0.2% Tryptamine

% Quantitation of actual* value using LC–MS/MS

 Ubiquitin

86.7 ± 8.5

100.8 ± 0.3

103.0 ± 0.7

 BSA

82.4 ± 0.8

92.7 ± 1.3

94.6 ± 0.5

 

H2O2 + 6 M HCl + β-ME

6 M HCl + β-ME

4 M MetS + 0.2% Tryptamine

% Quantitation of actual* value using AccQ-Tag

 Ubiquitin

79.6 ± 9.7

88.9 ± 0.5

48.8 ± 9.4

 BSA

80.5 ± 1.1

59.3 ± 7.9

14.6 ± 5.1

  1. Variability is expressed as standard error of mean (n = 3)
  2. H2O2 hydrogen peroxide, HCl hydrochloric acid, β-ME beta-mercaptoethanol, MetS methane sulfonic acid
  3. *Actual values are based on weighed amount of standards