Fig. 2

Sequence mutations and corresponding phenotypes of CRISPR/Cas9 transgenic lines of ‘Hawaii 4’. Examples of sequence mutations and allelic variants with corresponding phenotypes in transgenic shoot lines of Fragaria ‘Hawaii 4’ (line numbers 39 and 89). a Alignment of the sequence for each variant to the reference sequence. To the left of the panel, each sequence variant type is identified by the location of the mutation relative to the cut site:number bases deleted (D). Deletions result in amino acid loss (mutations − 1:3D and 1:3D), and also frameshift (mutation − 1:1D) and generation of stop codons within exon 7 resulting in truncated PDS protein. The cut site is shown by a black vertical line; the target site and PAM site (AGG) are within the box in the reference sequence. b Heat map showing the number and percentage of variant reads for each sample. Samples are arranged in columns and identified below the panel. c Images of phenotypes and an accompanying table giving descriptions for each sequenced sample. Numbers in the first column refer to the shoot line. In subsequent columns, individual shoots from a shoot line are designated using a letter (A, B, etc.) and numbers indicate individual leaves from individual shoots. White- or pale-green-box shading indicates albino or pale green/variegated phenotype, respectively, for shoots and leaves. WT = wild type. Scale bars are 5 mm