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Fig. 1 | Plant Methods

Fig. 1

From: CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry

Fig. 1

Schematic maps of CRISPR cassette, T-DNA of pCas9-K-GFP and Fragaria vesca PDS gene. a Single guide RNA inserted into the multiple cloning site (MCS) of pCas9-K-GFP. b pCas9-K-GFP T-DNA showing the relative position between the left border (LB) and right border (RB) of the MCS, the marker/selection expression cassettes 35S-GFP and Nos-Kan and the pcoCas9 coding sequence with the potato IV2 intron (IV2 Int), nuclear localistion signal (NLS), 35SPPDK promoter and Nos terminator. The binding sites  of primers used for TAIL-PCR (T1 = Tail 1; T2 = Tail 2), sgRNA insertion site PCR (mcs_F; mcs_R) and Nos-Kan cassette PCR (Nos-Kan F; Nos-Kan R) are indicated. c Fragaria vesca PDS gene, LG4-gene12690 (8035 bp, FvAssembly v4) showing exons (yellow arrows) and the location of the CRISPR target sequence PDS74 (position 3’–5’ = 3873–3892)

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