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Fig. 5 | Plant Methods

Fig. 5

From: Intact leaf gas exchange provides a robust method for measuring the kinetics of stomatal conductance responses to abscisic acid and other small molecules in Arabidopsis and grasses

Fig. 5

Aquaporin pip quadruple mutant is responsive to ABA. a Expression of PIP genes in WT (Col 0) and pip1;1, pip1;2, pip2;1 and pip2;2 mutants. Expression of tubulin 9 is shown as control. b Analyses of stomatal movements in response to ABA. Leaf epidermal layers were incubated with ± ABA (10 μM ABA) and the stomatal aperture was scored (N = 10 stomata per genotype per treatment). c Stomatal conductance of detached leaves from wild type (Col-0) (n = 3) and pip1;1/pip1;2/pip2;1/pip2;2 quadruple mutant (n = 4) plants in response to 2 μM ABA. d Normalized data shown in panel c. Data shown in c were normalized to the average of the first 10 min of stomatal conductance values recorded. ABA was added to the transpiration stream (red arrowhead). N > 3 leaves ± SEM per genotype

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Plant Methods

ISSN: 1746-4811