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Table 5 Ranking of candidate reference genes in order of their expression stability in the AR developmental stage

From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Ranking Genorm Normfinder BestKeeper Delta CT RankAggreg
Gene Stability Gene Stability Gene SD CV
1 EIF4A
PP2A-2
0.400 bHLH 0.422 miR482 0.33 1.34 PP2A-2 PP2A-2
2    UBP 0.424 PP2A-A2 0.45 1.41 ACT7 UBP
3 RPS18 0.432 PP2A-2 0.501 UBP 0.47 1.42 UBP bHLH
4 bHLH 0.612 U6-1 0.517 ATPase 0.48 1.61 bHLH PP2A-A2
5 UBP 0.669 PP2A-A2 0.576 ACT7 0.56 1.85 U6-1 ACT7
6 U6-1 0.704 U6-2 0.671 bHLH 0.61 2.03 PP2A-A2 miR482
7 ATPase 0.749 miR482 0.792 U6-1 0.67 2.08 miR482 U6-1
8 PP2A-A2 0.781 ACT7 0.830 PP2A-2 0.69 2.13 ATPase EIF4A
9 ACT7 0.804 EIF4A 0.931 RPS18 0.81 2.51 EIF4A ATPase
10 miR482 0.843 ATPase 0.934 Histone 0.83 2.73 U6-2 RPS18
11 Histone 0.877 Histone 0.951 U6-2 0.88 2.77 Histone U6-2
12 U6-2 0.904 RPS18 1.054 UBQ10 1.00 3.55 DNAJ Histone
13 DNAJ 0.941 DNAJ 1.078 DNAJ 1.03 3.23 UBQ10 DNAJ
14 GAPDH 0.963 UBQ10 1.160 EIF4A 1.04 3.32 RPS18 UBQ10
15 UBQ10 1.008 GAPDH 1.338 GAPDH 1.15 3.85 GAPDH GAPDH
16 miR171 1.062 miR403 1.508 miR403 1.22 4.54 miR403 miR403
17 miR403 1.136 miR171 1.979 miR171 1.34 6.68 miR171 miR171
18 5.8S 1.589 5.8S 4.693 5.8S 3.69 16.07 5.8S 5.8S