Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Table 5 Ranking of candidate reference genes in order of their expression stability in the AR developmental stage

Ranking	Genorm		Normfinder		BestKeeper			Delta CT	RankAggreg
Ranking	Gene	Stability	Gene	Stability	Gene	SD	CV	Delta CT	RankAggreg
1	EIF4A PP2A-2	0.400	bHLH	0.422	miR482	0.33	1.34	PP2A-2	PP2A-2
2			UBP	0.424	PP2A-A2	0.45	1.41	ACT7	UBP
3	RPS18	0.432	PP2A-2	0.501	UBP	0.47	1.42	UBP	bHLH
4	bHLH	0.612	U6-1	0.517	ATPase	0.48	1.61	bHLH	PP2A-A2
5	UBP	0.669	PP2A-A2	0.576	ACT7	0.56	1.85	U6-1	ACT7
6	U6-1	0.704	U6-2	0.671	bHLH	0.61	2.03	PP2A-A2	miR482
7	ATPase	0.749	miR482	0.792	U6-1	0.67	2.08	miR482	U6-1
8	PP2A-A2	0.781	ACT7	0.830	PP2A-2	0.69	2.13	ATPase	EIF4A
9	ACT7	0.804	EIF4A	0.931	RPS18	0.81	2.51	EIF4A	ATPase
10	miR482	0.843	ATPase	0.934	Histone	0.83	2.73	U6-2	RPS18
11	Histone	0.877	Histone	0.951	U6-2	0.88	2.77	Histone	U6-2
12	U6-2	0.904	RPS18	1.054	UBQ10	1.00	3.55	DNAJ	Histone
13	DNAJ	0.941	DNAJ	1.078	DNAJ	1.03	3.23	UBQ10	DNAJ
14	GAPDH	0.963	UBQ10	1.160	EIF4A	1.04	3.32	RPS18	UBQ10
15	UBQ10	1.008	GAPDH	1.338	GAPDH	1.15	3.85	GAPDH	GAPDH
16	miR171	1.062	miR403	1.508	miR403	1.22	4.54	miR403	miR403
17	miR403	1.136	miR171	1.979	miR171	1.34	6.68	miR171	miR171
18	5.8S	1.589	5.8S	4.693	5.8S	3.69	16.07	5.8S	5.8S

ISSN: 1746-4811