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Table 4 Expression stability ranking of the 18 candidate reference genes during plant regeneration

From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Method 1 2 3 4 5 6 7 8 9
(A) Ranking order across tissues at callus stage (better–good–average)
 Genorm U6-1|U6-2   EIF4A UBQ10 GAPDH ACT7 PP2A-2 ATPase Histone
 Normfinder Histone EIF4A U6-2 U6-1 PP2A-2 ACT7 ATPase UBQ10 GAPDH
 BestKeeper EIF4A U6-2 U6-1 UBQ10 Histone ACT7 ATPase GAPDH PP2A-2
 Delta CT EIF4A U6-2 U6-1 Histone PP2A-2 ATPase ACT7 UBQ10 GAPDH
 RankAggreg EIF4A U6-2 U6-1 Histone UBQ10 ACT7 PP2A-2 ATPase GAPDH
(B) Ranking order across tissues at seedlings stage (better–good–average)
 Genorm U6-1|U6-2   EIF4A RPS18 bHLH PP2A-2 ACT7 GAPDH UBP
 Normfinder U6-1 U6-2 EIF4A RPS18 bHLH GAPDH ACT7 PP2A-2 DNAJ
 BestKeeper Histone U6-1 EIF4A U6-2 RPS18 bHLH miR171 GAPDH PP2A-2
 Delta CT U6-2 U6-1 EIF4A PP2A-2 RPS18 bHLH ACT7 GAPDH UBP
 RankAggreg U6-1 U6-2 EIF4A RPS18 bHLH PP2A-2 ACT7 GAPDH DNAJ
(C) Ranking order across tissues at plants stage (better–good–average)
 Genorm PP2A-2|U6-1   RPS18 U6-2 EIF4A bHLH DNAJ UBP GAPDH
 Normfinder UBP bHLH DNAJ U6-1 PP2A-2 RPS18 PP2A-A2 UBQ10 EIF4A
 BestKeeper EIF4A U6-2 U6-1 Histone PP2A-2 GAPDH RPS18 bHLH PP2A-A2
 Delta CT PP2A-2 U6-1 bHLH UBP DNAJ RPS18 PP2A-A2 EIF4A GAPDH
 RankAggreg U6-1 PP2A-2 bHLH RPS18 EIF4A U6-2 UBP DNAJ PP2A-A2
(D) Ranking order under all samples (better–good–average)
 Genorm EIF4A|U6-1   U6-2 PP2A-2 bHLH PP2A-A2 UBP ATPase GAPDH
 Normfinder bHLH PP2A-2 UBP U6-1 U6-2 EIF4A GAPDH PP2A-A2 DNAJ
 BestKeeper EIF4A U6-1 Histone U6-2 PP2A-2 bHLH GAPDH UBP PP2A-A2
 Delta CT PP2A-2 U6-1 bHLH U6-2 UBP EIF4A PP2A-A2 GAPDH ATPase
 RankAggreg U6-1 EIF4A PP2A-2 U6-2 UBP PP2A-A2 bHLH GAPDH Histone
Method 10 11 12 13 14 15 16 17 18
(A) Ranking order across tissues at callus stage (better–good–average)
 Genorm PP2A-A2 RPS18 bHLH DNAJ UBP 5.8S miR482 miR403 miR171
 Normfinder RPS18 5.8S bHLH PP2A-A2 UBP DNAJ miR482 miR403 miR171
 BestKeeper PP2A-A2 bHLH 5.8S RPS18 DNAJ UBP miR482 miR403 miR171
 Delta CT PP2A-A2 RPS18 bHLH 5.8S DNAJ UBP miR482 miR403 miR171
 RankAggreg RPS18 PP2A-A2 bHLH 5.8S DNAJ UBP miR482 miR403 miR171
(B) Ranking order across tissues at seedlings stage (better–good–average)
 Genorm DNAJ PP2A-A2 ATPase UBQ10 Histone 5.8S miR171 miR403 miR482
 Normfinder UBP PP2A-A2 Histone UBQ10 ATPase 5.8S miR171 miR403 miR482
 BestKeeper DNAJ ACT7 UBQ10 UBP PP2A-A2 ATPase 5.8S miR403 miR482
 Delta CT DNAJ PP2A-A2 ATPase UBQ10 Histone 5.8S miR171 miR403 miR482
 RankAggreg UBP PP2A-A2 Histone UBQ10 ATPase 5.8S miR171 miR403 miR482
(C) Ranking order across tissues at plants stage (better–good–average)
 Genorm PP2A-A2 ATPase ACT7 UBQ10 Histone 5.8S miR171 miR482 miR403
 Normfinder Histone ACT7 U6-2 GAPDH ATPase 5.8S miR171 miR482 miR403
 BestKeeper DNAJ ATPase UBP ACT7 UBQ10 5.8S miR482 miR171 miR403
 Delta CT U6-2 ACT7 UBQ10 ATPase Histone 5.8S miR171 miR482 miR403
 RankAggreg GAPDH Histone ACT7 ATPase UBQ10 5.8S miR171 miR482 miR403
(D) Ranking order under all samples (better–good–average)
 Genorm ACT7 DNAJ Histone UBQ10 RPS18 5.8S miR482 miR171 miR403
 Normfinder Histone ATPase ACT7 RPS18 UBQ10 5.8S miR171 miR482 miR403
 BestKeeper ACT7 UBQ10 DNAJ ATPase RPS18 5.8S miR482 miR171 miR403
 Delta CT ACT7 DNAJ Histone UBQ10 RPS18 5.8S miR171 miR482 miR403
 RankAggreg ATPase ACT7 DNAJ UBQ10 RPS18 5.8S miR482 miR171 miR403