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Table 4 Expression stability ranking of the 18 candidate reference genes during plant regeneration

From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Method

1

2

3

4

5

6

7

8

9

(A) Ranking order across tissues at callus stage (better–good–average)

 Genorm

U6-1|U6-2

 

EIF4A

UBQ10

GAPDH

ACT7

PP2A-2

ATPase

Histone

 Normfinder

Histone

EIF4A

U6-2

U6-1

PP2A-2

ACT7

ATPase

UBQ10

GAPDH

 BestKeeper

EIF4A

U6-2

U6-1

UBQ10

Histone

ACT7

ATPase

GAPDH

PP2A-2

 Delta CT

EIF4A

U6-2

U6-1

Histone

PP2A-2

ATPase

ACT7

UBQ10

GAPDH

 RankAggreg

EIF4A

U6-2

U6-1

Histone

UBQ10

ACT7

PP2A-2

ATPase

GAPDH

(B) Ranking order across tissues at seedlings stage (better–good–average)

 Genorm

U6-1|U6-2

 

EIF4A

RPS18

bHLH

PP2A-2

ACT7

GAPDH

UBP

 Normfinder

U6-1

U6-2

EIF4A

RPS18

bHLH

GAPDH

ACT7

PP2A-2

DNAJ

 BestKeeper

Histone

U6-1

EIF4A

U6-2

RPS18

bHLH

miR171

GAPDH

PP2A-2

 Delta CT

U6-2

U6-1

EIF4A

PP2A-2

RPS18

bHLH

ACT7

GAPDH

UBP

 RankAggreg

U6-1

U6-2

EIF4A

RPS18

bHLH

PP2A-2

ACT7

GAPDH

DNAJ

(C) Ranking order across tissues at plants stage (better–good–average)

 Genorm

PP2A-2|U6-1

 

RPS18

U6-2

EIF4A

bHLH

DNAJ

UBP

GAPDH

 Normfinder

UBP

bHLH

DNAJ

U6-1

PP2A-2

RPS18

PP2A-A2

UBQ10

EIF4A

 BestKeeper

EIF4A

U6-2

U6-1

Histone

PP2A-2

GAPDH

RPS18

bHLH

PP2A-A2

 Delta CT

PP2A-2

U6-1

bHLH

UBP

DNAJ

RPS18

PP2A-A2

EIF4A

GAPDH

 RankAggreg

U6-1

PP2A-2

bHLH

RPS18

EIF4A

U6-2

UBP

DNAJ

PP2A-A2

(D) Ranking order under all samples (better–good–average)

 Genorm

EIF4A|U6-1

 

U6-2

PP2A-2

bHLH

PP2A-A2

UBP

ATPase

GAPDH

 Normfinder

bHLH

PP2A-2

UBP

U6-1

U6-2

EIF4A

GAPDH

PP2A-A2

DNAJ

 BestKeeper

EIF4A

U6-1

Histone

U6-2

PP2A-2

bHLH

GAPDH

UBP

PP2A-A2

 Delta CT

PP2A-2

U6-1

bHLH

U6-2

UBP

EIF4A

PP2A-A2

GAPDH

ATPase

 RankAggreg

U6-1

EIF4A

PP2A-2

U6-2

UBP

PP2A-A2

bHLH

GAPDH

Histone

Method

10

11

12

13

14

15

16

17

18

(A) Ranking order across tissues at callus stage (better–good–average)

 Genorm

PP2A-A2

RPS18

bHLH

DNAJ

UBP

5.8S

miR482

miR403

miR171

 Normfinder

RPS18

5.8S

bHLH

PP2A-A2

UBP

DNAJ

miR482

miR403

miR171

 BestKeeper

PP2A-A2

bHLH

5.8S

RPS18

DNAJ

UBP

miR482

miR403

miR171

 Delta CT

PP2A-A2

RPS18

bHLH

5.8S

DNAJ

UBP

miR482

miR403

miR171

 RankAggreg

RPS18

PP2A-A2

bHLH

5.8S

DNAJ

UBP

miR482

miR403

miR171

(B) Ranking order across tissues at seedlings stage (better–good–average)

 Genorm

DNAJ

PP2A-A2

ATPase

UBQ10

Histone

5.8S

miR171

miR403

miR482

 Normfinder

UBP

PP2A-A2

Histone

UBQ10

ATPase

5.8S

miR171

miR403

miR482

 BestKeeper

DNAJ

ACT7

UBQ10

UBP

PP2A-A2

ATPase

5.8S

miR403

miR482

 Delta CT

DNAJ

PP2A-A2

ATPase

UBQ10

Histone

5.8S

miR171

miR403

miR482

 RankAggreg

UBP

PP2A-A2

Histone

UBQ10

ATPase

5.8S

miR171

miR403

miR482

(C) Ranking order across tissues at plants stage (better–good–average)

 Genorm

PP2A-A2

ATPase

ACT7

UBQ10

Histone

5.8S

miR171

miR482

miR403

 Normfinder

Histone

ACT7

U6-2

GAPDH

ATPase

5.8S

miR171

miR482

miR403

 BestKeeper

DNAJ

ATPase

UBP

ACT7

UBQ10

5.8S

miR482

miR171

miR403

 Delta CT

U6-2

ACT7

UBQ10

ATPase

Histone

5.8S

miR171

miR482

miR403

 RankAggreg

GAPDH

Histone

ACT7

ATPase

UBQ10

5.8S

miR171

miR482

miR403

(D) Ranking order under all samples (better–good–average)

 Genorm

ACT7

DNAJ

Histone

UBQ10

RPS18

5.8S

miR482

miR171

miR403

 Normfinder

Histone

ATPase

ACT7

RPS18

UBQ10

5.8S

miR171

miR482

miR403

 BestKeeper

ACT7

UBQ10

DNAJ

ATPase

RPS18

5.8S

miR482

miR171

miR403

 Delta CT

ACT7

DNAJ

Histone

UBQ10

RPS18

5.8S

miR171

miR482

miR403

 RankAggreg

ATPase

ACT7

DNAJ

UBQ10

RPS18

5.8S

miR482

miR171

miR403