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Table 3 Ranking of the expression stability of candidate reference genes calculated by BestKeeper during plant regeneration

From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Rank Callus Seedlings Plants Total
Gene SD CV Gene SD CV Gene SD CV Gene SD CV
1 EIF4A 0.07 0.28 Histone 0.27 1.14 EIF4A 0.43 1.63 EIF4A 0.37 1.39
2 U6-2 0.08 0.31 U6-1 0.28 1 U6-2 0.49 1.77 U6-1 0.39 1.38
3 U6-1 0.12 0.41 EIF4A 0.28 1.09 U6-1 0.52 1.83 Histone 0.39 1.65
4 UBQ10 0.15 0.65 U6-2 0.29 1.09 Histone 0.53 2.2 U6-2 0.44 1.6
5 Histone 0.16 0.65 RPS18 0.37 1.22 PP2A-2 0.54 1.99 PP2A-2 0.49 1.79
6 ACT7 0.17 0.65 bHLH 0.4 1.48 GAPDH 0.58 2.4 bHLH 0.61 2.21
7 ATPase 0.17 0.63 miR171 0.44 1.91 RPS18 0.6 1.94 GAPDH 0.61 2.54
8 GAPDH 0.19 0.79 GAPDH 0.45 1.89 bHLH 0.69 2.48 UBP 0.66 2.1
9 PP2A-2 0.19 0.69 PP2A-2 0.49 1.82 PP2A-A2 0.71 2.53 PP2A-A2 0.66 2.38
10 PP2A-A2 0.32 1.15 DNAJ 0.49 1.96 DNAJ 0.71 2.65 ACT7 0.7 2.64
11 bHLH 0.33 1.22 ACT7 0.56 2.19 ATPase 0.75 2.74 UBQ10 0.7 3.1
12 5.8S 0.33 1.5 UBQ10 0.57 2.55 UBP 0.76 2.39 DNAJ 0.71 2.69
13 RPS18 0.34 1.15 UBP 0.6 1.94 ACT7 0.81 3.08 ATPase 0.74 2.7
14 DNAJ 0.42 1.62 PP2A-A2 0.6 2.21 UBQ10 0.84 3.74 RPS18 0.76 2.48
15 UBP 0.44 1.4 ATPase 0.63 2.36 5.8S 1.09 5.03 5.8S 0.93 4.25
16 miR482 0.48 2.39 5.8S 0.79 3.62 miR482 1.27 6.11 miR482 1.11 5.39
17 miR403 0.64 2.75 miR403 0.83 3.36 miR171 1.82 7.68 miR171 1.48 6.39
18 miR171 0.88 4.14 miR482 1.22 5.8 miR403 1.97 7.62 miR403 1.63 6.47