Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Table 3 Ranking of the expression stability of candidate reference genes calculated by BestKeeper during plant regeneration

Rank	Callus			Seedlings			Plants			Total
Rank	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV
1	EIF4A	0.07	0.28	Histone	0.27	1.14	EIF4A	0.43	1.63	EIF4A	0.37	1.39
2	U6-2	0.08	0.31	U6-1	0.28	1	U6-2	0.49	1.77	U6-1	0.39	1.38
3	U6-1	0.12	0.41	EIF4A	0.28	1.09	U6-1	0.52	1.83	Histone	0.39	1.65
4	UBQ10	0.15	0.65	U6-2	0.29	1.09	Histone	0.53	2.2	U6-2	0.44	1.6
5	Histone	0.16	0.65	RPS18	0.37	1.22	PP2A-2	0.54	1.99	PP2A-2	0.49	1.79
6	ACT7	0.17	0.65	bHLH	0.4	1.48	GAPDH	0.58	2.4	bHLH	0.61	2.21
7	ATPase	0.17	0.63	miR171	0.44	1.91	RPS18	0.6	1.94	GAPDH	0.61	2.54
8	GAPDH	0.19	0.79	GAPDH	0.45	1.89	bHLH	0.69	2.48	UBP	0.66	2.1
9	PP2A-2	0.19	0.69	PP2A-2	0.49	1.82	PP2A-A2	0.71	2.53	PP2A-A2	0.66	2.38
10	PP2A-A2	0.32	1.15	DNAJ	0.49	1.96	DNAJ	0.71	2.65	ACT7	0.7	2.64
11	bHLH	0.33	1.22	ACT7	0.56	2.19	ATPase	0.75	2.74	UBQ10	0.7	3.1
12	5.8S	0.33	1.5	UBQ10	0.57	2.55	UBP	0.76	2.39	DNAJ	0.71	2.69
13	RPS18	0.34	1.15	UBP	0.6	1.94	ACT7	0.81	3.08	ATPase	0.74	2.7
14	DNAJ	0.42	1.62	PP2A-A2	0.6	2.21	UBQ10	0.84	3.74	RPS18	0.76	2.48
15	UBP	0.44	1.4	ATPase	0.63	2.36	5.8S	1.09	5.03	5.8S	0.93	4.25
16	miR482	0.48	2.39	5.8S	0.79	3.62	miR482	1.27	6.11	miR482	1.11	5.39
17	miR403	0.64	2.75	miR403	0.83	3.36	miR171	1.82	7.68	miR171	1.48	6.39
18	miR171	0.88	4.14	miR482	1.22	5.8	miR403	1.97	7.62	miR403	1.63	6.47

ISSN: 1746-4811