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Table 2 Ranking of the expression stability of candidate reference genes calculated using NormFinder during plant regeneration

From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Rank Callus Seedlings Plants Total
Gene Stability Gene Stability Gene Stability Gene Stability
1 Histone 0.041 U6-1 0.041 UBP 0.210 bHLH 0.275
2 EIF4A 0.168 U6-2 0.041 bHLH 0.228 PP2A-2 0.302
3 U6-2 0.213 EIF4A 0.043 DNAJ 0.247 U6-1 0.303
4 U6-1 0.221 RPS18 0.082 U6-1 0.288 UBP 0.308
5 PP2A-2 0.222 bHLH 0.151 PP2A-2 0.296 U6-2 0.376
6 ACT7 0.277 GAPDH 0.267 RPS18 0.380 EIF4A 0.402
7 ATPase 0.284 ACT7 0.282 PP2A-A2 0.418 GAPDH 0.419
8 UBQ10 0.310 PP2A-2 0.288 UBQ10 0.424 PP2A-A2 0.427
9 GAPDH 0.335 DNAJ 0.374 EIF4A 0.427 DNAJ 0.461
10 RPS18 0.417 UBP 0.392 Histone 0.440 Histone 0.485
11 5.8S 0.424 PP2A-A2 0.472 ACT7 0.468 ATPase 0.499
12 bHLH 0.428 Histone 0.508 U6-2 0.471 ACT7 0.521
13 PP2A-A2 0.441 UBQ10 0.558 GAPDH 0.471 RPS18 0.561
14 UBP 0.526 ATPase 0.575 ATPase 0.544 UBQ10 0.582
15 DNAJ 0.533 5.8S 0.855 5.8S 0.921 5.8S 0.967
16 miR482 0.712 miR171 0.901 miR171 1.405 miR171 1.473
17 miR403 0.773 miR403 1.498 miR482 1.618 miR482 1.506
18 miR171 1.288 miR482 1.710 miR403 1.852 miR403 1.767