Skip to main content

Table 1 The description of candidate reference genes and primers used in this study

From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

Gene symbol

Gene name

Gene ID

Arabidopsis homolog

Forward primer sequence (5′–3′)

Reverse primer sequence (5′–3′)

Size (bp)

E (%)

R2

ACT7

Actin 7

Potri.001G309500

AT5G09810

GCATCCACGAGACTACATACAACTCA

GTGATCTCCTTGCTCATTCGGTCA

136

100.02

0.998

EIF4A

Eukaryotic initiation factor 4A

Potri.005G093900

AT3G19760

TACATTCATCGAATTGGTCGTTCTGGT

TTCATAGGCATTTCGTCAATCTGGG

137

101.50

0.995

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Potri.012G094100

AT1G13440

AACCGACTTCATTGGTGACAACCG

CCACTCATTGTCATACCACGCAAC

106

100.72

0.997

Histone

Histone superfamily protein

Potri.005G072300

AT4G40030

ACTGTTGCTCTTCGTGAAATCCGTA

CTTAAAATCCTGGGCAATTTCACGAAC

105

96.62

0.998

PP2A-2

Protein phosphatase 2A-2

Potri.015G068300

AT1G10430

ACAGTTCAACCACACTAATGGGCTC

TTTGGCGCACTGAACACTGTAACCAC

114

103.41

0.988

PP2A-A2

Protein phosphatase 2A subunit A2

Potri.010G127500

AT3G25800

ATGAATTTCCTGATGTGCGACT

CAATGCCTATCCTCTGCAAGCTC

127

99.23

0.998

RPS18

Ribosomal protein S18

Potri.006G170500

AT1G07210

AGGCTCATCATCTTATCAAATCCCT

TCAATGCCACCAAATATTCGTTGCT

127

103.69

0.990

UBQ10

Polyubiquitin 10

Potri.001G418500

AT4G05320

GTTGATTTTTGCTGGGAAGC

GATCTTGGCCTTCACGTTGT

192

98.18

1.000

ATPase

ATP synthase subunit B

Potri.004G177500

AT4G38510

ACTCATCCCACCCCTGATCTTACGG

ACCAATGGCACTCTTCATGAGACGA

138

101.65

0.995

UBP

Oligouridylate binding protein 1B

Potri.006G279600

AT1G17370

GGCTTTGTTTCATTCCGTAATCAGCA

AACACCTTTAGTTGCCCAATTGCAT

111

100.92

0.978

bHLH

bHLH transcription factor

Potri.011G132400

AT5G54680

ATCTGAATCGTGTAGTGCGTCTAGCTC

GCATCAACCAAAATAGCAGCCTTGTCC

147

101.61

0.985

DNAJ

DNAJ homologue 2

Potri.010G243100

AT5G22060

AGGCAATTAATGACAAGGACCGTT

AGCCTCTCCAGGGAAAGTAATCCTC

131

101.98

0.996

U6-1

Small nuclear ribonucleoprotein family protein

Potri.001G166600

AT3G14080

GTGACCTTTATTGCGACATCCACT

CTTCTGAAACACGAGTCATATGTGGT

123

96.16

0.995

U6-2

Small nuclear ribonucleoprotein family protein

Potri.008G078400

AT2G43810

GCCTGTTGTGGTTAAGCTCAATTCTG

TTCCTCGTATGAAAGCATCACCAT

149

99.12

0.999

5.8s

5.8S ribosomal RNA gene

AJ006440

 

ACGTCTGCCTGGGTGTCAC

TCAACCACCGCTCGTCGTG

145

108.37

0.993

miR171

MIMAT0001985

ptc-miR171c

 

AGATTGAGCCGCGCCAATATC

AACGAGACGACGACAGACTTT

49

107.44

0.967

miR403

MIMAT0002056

ptc-miR403a

 

CGCGTTAGATTCACGCACAAACTC

AACGAGACGACGACAGACTTT

57

115.04

0.982

miR482

MIMAT0002103

ptc-miR482a.1

 

CCTACTCCTCCCATTCCAAAA

AACGAGACGACGACAGACTTT

50

116.69

0.941