Table 1 Candidate reference genes and target genes description and primer sequences
Gene abbreviation | Gene name | Primer sequences (5′-3′) (forward/reverse) | Amplicon product | E (%) Amplification efficiency | R2 Correlation coefficients | |
|---|---|---|---|---|---|---|
Tm (°C) | Length (bp) | |||||
ACT | Actin7 | CAGATGTGGATTAGCAAG/CAAGCCGTAGTAGGTAAT | 79.5 | 99 | 92.2 | 0.988 |
UBQ | Ubiquitin 10 | CTTGAGGATGGTCGCACCTT/GTCGGAGCTTTCCACTTCCA | 83.7 | 144 | 98.6 | 0.998 |
NAC | NAC domain-containing protein | AGGCGTGAAGAAAGTCCTGG/CGGTACTCGTGCATGATCCA | 82.7 | 81 | 98.3 | 0.993 |
F-box | F-box family protein | AAGAGAACAGTGGTAGAC/TTGGTAGCAGGATTAAGG | 81.8 | 104 | 101.3 | 0.992 |
PP2C | Protein phosphatase 2C | CGACTGATAATGGAATGG/CGGAAACTGTAGGTACTA | 82.0 | 123 | 98.8 | 0.999 |
TUA | Alpha-tubulin | TCAGTGGATTATGGCAAGA/TAGAAGGGAATGGGTTGAC | 76.6 | 114 | 97.5 | 0.990 |
TUB | Beta-tubulin | CTCACAGCAATACATAGCC/TCAGCAGCACACATCATA | 78.8 | 81 | 91.1 | 0.998 |
UBC | Ubiquitin-conjugating Enzyme E2 | CTCTACCAATCCACCAGTT/GCAAGCCACCAGATAATG | 77.8 | 100 | 100.9 | 0.998 |
18S | 18S ribosomal RNA | CAATGGCAATGACAATGG/CAGAGAACAATCCGAACT | 81.7 | 75 | 95.1 | 0.999 |
ChNCS | Norcoclaurine synthase | AGAGGTAATGAGCAATGG/TGAGGCGTCTACAAGTTC | 82.5 | 143 | 98.5 | 0.996 |