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Fig. 6 | Plant Methods

Fig. 6

From: Development of an innovative and sustainable one-step method for rapid plant DNA isolation for targeted PCR using magnetic ionic liquids

Fig. 6

PCR amplification of the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequence (a) and the intergenic spacers of the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RbcL) gene (b) from A. thaliana, after extraction with the commercial kit (lane 2), the Ni-containing MIL (lanes 3 and 4), and the Co-containing MIL (lane 5 and 6). The 1 Kb DNA ladder marker is shown in lane 1. Extraction conditions: 6 µl of MIL, extraction time: 30 s

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Plant Methods

ISSN: 1746-4811