Fig. 1

Schematic representation of the enhancer validation pipeline using a luciferase-based transient assay. a Candidate enhancers were predicted based on DHSs and other chromatin datasets. b The predicted enhancer sequence was synthesized and cloned in the pCAMBIA-CRE-LUC vector containing a mini 35S promoter and the firefly luciferase reporter gene, and was transferred into Agrobacterium strain GV3101. c Each construct was agroinfiltrated into N. benthamiana leaves together with both positive and negative controls. d Bioluminescent data were collected using the NightSHADE LB 985 plant imaging system