Table 1 Labeling reagents, commercial source and dilution guide
Labeling reagent | Company/catalogue number | Dilution (diluent) |
|---|---|---|
Rat monoclonal anti-α tubulin antibody (clone YOL1/34) | BioRad/MCA78G | 1:300 (3% w/v BSA in PBS pH 7.3) |
Alpaca anti-GFP nanobody-Atto 488 conjugated (GFP-Booster) | Chromotek/gba488 | 1:500 (3% w/v BSA and 0.5% w/v BSAc in PBS pH 7.4) |
Rabbit polyclonal anti-MPK6 antibody | Sigma/A7104 | 1:750 (3% w/v BSA in PBS pH 7. 3) |
Rabbit polyclonal anti-pERK antibody | Cell Signaling/9101 s | 1:400 (3% w/v BSA in PBS pH 7.3) |
Goat anti-rat IgGs-Alexa Fluor 488 conjugated | ThermoFischer Scientific/A11006 | 1:500 (3% w/v BSA in PBS pH 7.3) |
Goat anti-rat IgGs-Alexa Fluor 647 conjugated | ThermoFischer Scientific/A21247 | 1:500 (3% w/v BSA in PBS pH 7.3) |
Goat anti-rabbit IgGs-Alexa Fluor 546 conjugated | ThermoFischer Scientific/A11010 | 1:1000 (3% w/v BSA and 0.5% w/v BSAc in PBS pH 7.4) |
Goat anti-mouse IgGs-Alexa Fluor 555 conjugated | ThermoFischer Scientific/A21422 | 1:500 (3% w/v BSA 3 in PBS pH 7.3) |