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Table 1 Labeling reagents, commercial source and dilution guide

From: Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins

Labeling reagent Company/catalogue number Dilution (diluent)
Rat monoclonal anti-α tubulin antibody (clone YOL1/34) BioRad/MCA78G 1:300 (3% w/v BSA in PBS pH 7.3)
Alpaca anti-GFP nanobody-Atto 488 conjugated (GFP-Booster) Chromotek/gba488 1:500 (3% w/v BSA and 0.5% w/v BSAc in PBS pH 7.4)
Rabbit polyclonal anti-MPK6 antibody Sigma/A7104 1:750 (3% w/v BSA in PBS pH 7. 3)
Rabbit polyclonal anti-pERK antibody Cell Signaling/9101 s 1:400 (3% w/v BSA in PBS pH 7.3)
Goat anti-rat IgGs-Alexa Fluor 488 conjugated ThermoFischer Scientific/A11006 1:500 (3% w/v BSA in PBS pH 7.3)
Goat anti-rat IgGs-Alexa Fluor 647 conjugated ThermoFischer Scientific/A21247 1:500 (3% w/v BSA in PBS pH 7.3)
Goat anti-rabbit IgGs-Alexa Fluor 546 conjugated ThermoFischer Scientific/A11010 1:1000 (3% w/v BSA and 0.5% w/v BSAc in PBS pH 7.4)
Goat anti-mouse IgGs-Alexa Fluor 555 conjugated ThermoFischer Scientific/A21422 1:500 (3% w/v BSA 3 in PBS pH 7.3)
  1. A list of primary and secondary antibodies used in the immunolocalizations of the present study, information on their commercial availability and their dilutions used herein