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Fig. 6 | Plant Methods

Fig. 6

From: Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins

Fig. 6

2-color 3D-SIM of MAP65-3 and microtubule distribution in cytokinesis. a Clockwise 3D rendering of MAP65-3, microtubules and their overlay in an early phragmoplast wherein both signals occupy the entire surface of the phragmoplast (corresponding to Additional file 4: Video 4). bd Maximum intensity projection showing microtubule (b), MAP65-3 (c) and their overlay (d) in the same phragmoplast as in a. e Clockwise 3D rendering of MAP65-3, microtubules and their overlay in an late phragmoplast wherein both signals are restricted in the edges of the phragmoplast (corresponding to Additional file 5: Video 5). fh Maximum intensity projection showing microtubule (f), MAP65-3 (g) and their overlay (h) in the same phragmoplast as in e. Bars = 2 μm

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