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Fig. 5 | Plant Methods

Fig. 5

From: Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins

Fig. 5

Differential localization of eGFP-MAP65-3 with mitotic and cytokinetic microtubule systems. a Microtubule, MAP65-3 and overlay of a metaphase spindle (top bracket) devoid of MAP65-3 signal and a telophase spindle (arrow) showing broad accumulation of MAP65-3 at the midplane (arrowheads). b Colocalization of microtubules and MAP65-3 at the midzone (arrowheads). c A more advanced phragmoplast with similar midplane distribution of MAP65-3 (arrowheads). d Restriction of MAP65-3 signal at phragmoplast edges (arrowheads) and its disappearance from central areas of the phragmoplast devoid of microtubules. Bars in a–d = 10 μm

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Plant Methods

ISSN: 1746-4811