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Fig. 4 | Plant Methods

Fig. 4

From: Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula

Fig. 4

Steps in Tissue Culture Protocol 2A/B. a Initial seedlings used as source. b Dissection of root into segments. c Calli induced after 4 or 6 weeks on CTM3 or RCTM3. d Calli after 1 week on RCTM4. e Embryos forming after 4 weeks on RCTM4. f Shoot forming after 1 week on RCTM5. g, h Shoot developed and root started to differentiate after 4 weeks on RCTM5. i Root (arrow) developing after 3 weeks on RCTM6

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