Fig. 5

Down-regulation of E_rtip1-dependent root-tip GFP expression by five environmental cues within 24 h. Growth of transgenic plants harboring P_{Ertip1+35Smini}:GFP, microscopic observation and quantification of fluorescence signals derived from GFP are described as that in Fig. 4. a Representative image of green fluorescence signals from GFP in the root apex. Pictures were taken after exposure of plants to the treatments i.e. cold (4 °C), NH₄⁺ (10 mM), salt (80 mM), P (2.5 mM) and pH 8 for 0 h, 1 h, 6 h, 12 h or 24 h. Bars = 50 μm. b–g Quantification of green fluorescence intensity in the root apex of plants without the treatment (CK) or with the treatment of cold (4 °C), NH₄⁺ (10 mM), Na⁺ (80 mM), P (2.5 mM) or pH 8. Means of 6 biological replicates ± SD (n = 6) were plotted and different letters above the bars indicate statistically significant differences (P < 0.05 by one-way ANOVA and a multiple comparison test)