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Table 2 Expression stability rankings of candidate reference genes evaluated by ΔCt, BestKeeper, NormFinder, geNorm, and RefFinder

From: Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

Method Expression stability Ranking order (1st is the most stable and 11th is the least stable)
RPL4 RPL18 HIS3 TUA3 SAMDC TIP41 UGPase 18S GAPDH PIP2 ACT
All samples
Delta CT 1.36 (1) 1.36 (2) 1.37 (3) 1.38 (4) 2.20 (11) 1.46 (6) 1.53 (8) 1.63 (9) 1.49 (7) 1.83 (10) 1.40 (5)
BestKeeper 1.03 (5) 0.98 (3) 0.87 (1) 0.91 (2) 1.50 (11) 1.01 (4) 1.06 (6) 1.29 (9) 1.25 (8) 1.43 (10) 1.10 (7)
NormFinder 0.79 (2) 0.84 (4) 0.80 (3) 0.78 (1) 1.99 (11) 1.02 (7) 1.05 (8) 1.25 (9) 1.02 (6) 1.51 (10) 0.87 (5)
geNorm 0.80 (1) 0.80 (1) 1.00 (4) 1.13 (6) 1.55 (11) 0.89 (3) 1.19 (7) 1.31 (9) 1.24 (8) 1.40 (10) 1.09 (5)
Recommended comprehensive ranking 1.78 (1) 2.21 (2) 2.45 (3) 2.63 (4) 11.00 (11) 4.74 (5) 7.20 (7) 9.00 (9) 7.20 (8) 10.00 (10) 5.44 (6)
Fruit
Delta CT 0.83 (5) 0.67 (1) 0.85 (6) 0.86 (7) 1.48 (11) 0.83 (4) 1.02 (9) 0.82 (3) 0.72 (2) 1.10 (10) 0.89 (8)
BestKeeper 0.43 (6) 0.40 (3) 0.79 (8) 0.43 (5) 0.94 (11) 0.83 (9) 0.31 (2) 0.50 (7) 0.22 (1) 0.41 (4) 0.86 (10)
NormFinder 0.54 (4) 0.18 (1) 0.59 (7) 0.57 (5) 1.37 (11) 0.59 (6) 0.78 (9) 0.53 (3) 0.27 (2) 0.94 (10) 0.66 (8)
geNorm 0.61 (7) 0.43 (4) 0.31 (3) 0.64 (8) 0.92 (11) 0.20 (1) 0.73 (9) 0.53 (5) 0.59 (6) 0.79 (10) 0.20 (1)
Recommended comprehensive ranking 5.38 (6) 1.86 (1) 5.63 (7) 6.12 (8) 11.00 (11) 3.83 (3) 6.18 (9) 4.21 (4) 2.21 (2) 7.95 (10) 5.03 (5)
Floral tissues
Delta CT 1.65 (5) 1.40 (2) 1.44 (3) 1.71 (8) 2.41 (11) 1.70 (7) 1.31 (1) 1.79 (9) 1.67 (6) 1.95 (10) 1.55 (4)
BestKeeper 1.87 (10) 1.18 (2) 0.82 (1) 1.69 (9) 2.57 (11) 1.58 (6) 1.33 (1) 1.20 (3) 1.64 (8) 1.59 (7) 1.56 (5)
NormFinder 1.16 (5) 0.79 (2) 0.81 (3) 1.22 (7) 2.21 (11) 1.32 (8) 0.36 (1) 1.41 (9) 1.19 (6) 1.58 (10) 0.95 (4)
geNorm 1.02 (5) 0.59 (1) 0.59 (1) 1.45 (9) 1.69 (11) 0.93 (4) 0.77 (3) 1.27 (7) 1.16 (6) 1.53 (10) 1.36 (8)
Recommended comprehensive ranking 5.95 (5) 1.68 (1) 1.73 (2) 8.21 (9) 11.00 (11) 6.05 (6) 1.86 (3) 6.42 (7) 6.45 (8) 9.15 (10) 5.03 (4)
Ovule and seed
Delta CT 0.95 (8) 0.95 (7) 1.07 (9) 0.76 (2) 0.91 (5) 0.74 (1) 1.31 (10) 0.90 (4) 0.92 (6) 1.44 (11) 0.78 (3)
BestKeeper 0.62 (7) 0.40 (3) 0.79 (9) 0.49 (4) 0.30 (1) 0.34 (2) 0.91 (10) 0.56 (6) 0.73 (8) 1.09 (11) 0.54 (5)
NormFinder 0.73 (8) 0.70 (7) 0.87 (9) 0.16 (2) 0.60 (6) 0.12 (1) 1.22 (10) 0.59 (5) 0.57 (4) 1.37 (11) 0.27 (3)
geNorm 0.56 (5) 0.49 (3) 0.79 (9) 0.54 (4) 0.38 (1) 0.38 (1) 0.87 (10) 0.73 (8) 0.68 (7) 0.98 (11) 0.62 (6)
Recommended comprehensive ranking 6.88 (8) 4.58 (5) 9.00 (9) 2.83 (3) 2.34 (2) 1.19 (1) 10.00 (10) 5.57 (6) 6.05 (7) 11.00 (11) 4.05 (4)
Vegetative tissues
Delta CT 0.83 (2) 0.78 (1) 1.08 (7) 0.92 (4) 1.43 (11) 0.91 (3) 1.01 (5) 1.22 (9) 1.23 (10) 1.18 (8) 1.03 (6)
BestKeeper 0.69 (4) 0.51 (1) 0.89 (5) 0.61 (2) 1.03 (9) 0.91 (6) 1.00 (8) 1.12 (11) 0.99 (7) 0.65 (3) 1.06 (10)
NormFinder 0.32 (2) 0.20 (1) 0.83 (7) 0.51 (3) 1.26 (11) 0.60 (4) 0.67 (5) 0.97 (9) 1.01 (10) 0.95 (8) 0.80 (6)
geNorm 0.47 (3) 0.54 (4) 0.65 (6) 0.62 (5) 1.06 (11) 0.21 (1) 0.74 (7) 0.97 (10) 0.91 (9) 0.84 (8) 0.21 (1)
Recommended comprehensive ranking 2.63 (2) 1.41 (1) 6.19 (7) 3.31 (4) 10.46 (11) 2.91 (3) 6.12 (6) 9.72 (10) 8.91 (9) 6.26 (8) 4.36 (5)
  1. Values in Delta CT are the average STDEV and CV ± SD values for BestKeeper (CV, the coefficient of variance; SD standard deviation). Values in geNorm are expression stability values (M value), and values in NormFinder are stability values (SVs). Ranking values in RefFinder are geometric means of ranking values of the four computational programs. Values in brackets show the ranking orders of each gene