Table 2 Expression stability rankings of candidate reference genes evaluated by ΔCt, BestKeeper, NormFinder, geNorm, and RefFinder
Method | Expression stability Ranking order (1st is the most stable and 11th is the least stable) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
RPL4 | RPL18 | HIS3 | TUA3 | SAMDC | TIP41 | UGPase | 18S | GAPDH | PIP2 | ACT | |
All samples | |||||||||||
Delta CT | 1.36 (1) | 1.36 (2) | 1.37 (3) | 1.38 (4) | 2.20 (11) | 1.46 (6) | 1.53 (8) | 1.63 (9) | 1.49 (7) | 1.83 (10) | 1.40 (5) |
BestKeeper | 1.03 (5) | 0.98 (3) | 0.87 (1) | 0.91 (2) | 1.50 (11) | 1.01 (4) | 1.06 (6) | 1.29 (9) | 1.25 (8) | 1.43 (10) | 1.10 (7) |
NormFinder | 0.79 (2) | 0.84 (4) | 0.80 (3) | 0.78 (1) | 1.99 (11) | 1.02 (7) | 1.05 (8) | 1.25 (9) | 1.02 (6) | 1.51 (10) | 0.87 (5) |
geNorm | 0.80 (1) | 0.80 (1) | 1.00 (4) | 1.13 (6) | 1.55 (11) | 0.89 (3) | 1.19 (7) | 1.31 (9) | 1.24 (8) | 1.40 (10) | 1.09 (5) |
Recommended comprehensive ranking | 1.78 (1) | 2.21 (2) | 2.45 (3) | 2.63 (4) | 11.00 (11) | 4.74 (5) | 7.20 (7) | 9.00 (9) | 7.20 (8) | 10.00 (10) | 5.44 (6) |
Fruit | |||||||||||
Delta CT | 0.83 (5) | 0.67 (1) | 0.85 (6) | 0.86 (7) | 1.48 (11) | 0.83 (4) | 1.02 (9) | 0.82 (3) | 0.72 (2) | 1.10 (10) | 0.89 (8) |
BestKeeper | 0.43 (6) | 0.40 (3) | 0.79 (8) | 0.43 (5) | 0.94 (11) | 0.83 (9) | 0.31 (2) | 0.50 (7) | 0.22 (1) | 0.41 (4) | 0.86 (10) |
NormFinder | 0.54 (4) | 0.18 (1) | 0.59 (7) | 0.57 (5) | 1.37 (11) | 0.59 (6) | 0.78 (9) | 0.53 (3) | 0.27 (2) | 0.94 (10) | 0.66 (8) |
geNorm | 0.61 (7) | 0.43 (4) | 0.31 (3) | 0.64 (8) | 0.92 (11) | 0.20 (1) | 0.73 (9) | 0.53 (5) | 0.59 (6) | 0.79 (10) | 0.20 (1) |
Recommended comprehensive ranking | 5.38 (6) | 1.86 (1) | 5.63 (7) | 6.12 (8) | 11.00 (11) | 3.83 (3) | 6.18 (9) | 4.21 (4) | 2.21 (2) | 7.95 (10) | 5.03 (5) |
Floral tissues | |||||||||||
Delta CT | 1.65 (5) | 1.40 (2) | 1.44 (3) | 1.71 (8) | 2.41 (11) | 1.70 (7) | 1.31 (1) | 1.79 (9) | 1.67 (6) | 1.95 (10) | 1.55 (4) |
BestKeeper | 1.87 (10) | 1.18 (2) | 0.82 (1) | 1.69 (9) | 2.57 (11) | 1.58 (6) | 1.33 (1) | 1.20 (3) | 1.64 (8) | 1.59 (7) | 1.56 (5) |
NormFinder | 1.16 (5) | 0.79 (2) | 0.81 (3) | 1.22 (7) | 2.21 (11) | 1.32 (8) | 0.36 (1) | 1.41 (9) | 1.19 (6) | 1.58 (10) | 0.95 (4) |
geNorm | 1.02 (5) | 0.59 (1) | 0.59 (1) | 1.45 (9) | 1.69 (11) | 0.93 (4) | 0.77 (3) | 1.27 (7) | 1.16 (6) | 1.53 (10) | 1.36 (8) |
Recommended comprehensive ranking | 5.95 (5) | 1.68 (1) | 1.73 (2) | 8.21 (9) | 11.00 (11) | 6.05 (6) | 1.86 (3) | 6.42 (7) | 6.45 (8) | 9.15 (10) | 5.03 (4) |
Ovule and seed | |||||||||||
Delta CT | 0.95 (8) | 0.95 (7) | 1.07 (9) | 0.76 (2) | 0.91 (5) | 0.74 (1) | 1.31 (10) | 0.90 (4) | 0.92 (6) | 1.44 (11) | 0.78 (3) |
BestKeeper | 0.62 (7) | 0.40 (3) | 0.79 (9) | 0.49 (4) | 0.30 (1) | 0.34 (2) | 0.91 (10) | 0.56 (6) | 0.73 (8) | 1.09 (11) | 0.54 (5) |
NormFinder | 0.73 (8) | 0.70 (7) | 0.87 (9) | 0.16 (2) | 0.60 (6) | 0.12 (1) | 1.22 (10) | 0.59 (5) | 0.57 (4) | 1.37 (11) | 0.27 (3) |
geNorm | 0.56 (5) | 0.49 (3) | 0.79 (9) | 0.54 (4) | 0.38 (1) | 0.38 (1) | 0.87 (10) | 0.73 (8) | 0.68 (7) | 0.98 (11) | 0.62 (6) |
Recommended comprehensive ranking | 6.88 (8) | 4.58 (5) | 9.00 (9) | 2.83 (3) | 2.34 (2) | 1.19 (1) | 10.00 (10) | 5.57 (6) | 6.05 (7) | 11.00 (11) | 4.05 (4) |
Vegetative tissues | |||||||||||
Delta CT | 0.83 (2) | 0.78 (1) | 1.08 (7) | 0.92 (4) | 1.43 (11) | 0.91 (3) | 1.01 (5) | 1.22 (9) | 1.23 (10) | 1.18 (8) | 1.03 (6) |
BestKeeper | 0.69 (4) | 0.51 (1) | 0.89 (5) | 0.61 (2) | 1.03 (9) | 0.91 (6) | 1.00 (8) | 1.12 (11) | 0.99 (7) | 0.65 (3) | 1.06 (10) |
NormFinder | 0.32 (2) | 0.20 (1) | 0.83 (7) | 0.51 (3) | 1.26 (11) | 0.60 (4) | 0.67 (5) | 0.97 (9) | 1.01 (10) | 0.95 (8) | 0.80 (6) |
geNorm | 0.47 (3) | 0.54 (4) | 0.65 (6) | 0.62 (5) | 1.06 (11) | 0.21 (1) | 0.74 (7) | 0.97 (10) | 0.91 (9) | 0.84 (8) | 0.21 (1) |
Recommended comprehensive ranking | 2.63 (2) | 1.41 (1) | 6.19 (7) | 3.31 (4) | 10.46 (11) | 2.91 (3) | 6.12 (6) | 9.72 (10) | 8.91 (9) | 6.26 (8) | 4.36 (5) |