Selection of the optimal reference genes for expression analyses in different materials of Eriobotrya japonica

Table 1 Reference gene primer sequences and amplicon characteristics for each primer pair used in the RT-qPCR

Gene	Gene description	GenBank ID	Primer sequence (5′–3′)	Amplicon length (bp)	Amplicon Tm (°C)	Amplification efficiency (%)	Regression coefficient (R²⁾
RPL4	Ribosomal protein L4	MH196506	F: AGGTTCAGTCAGTCGTCAGGC	215	81.59	102.91	0.994
			R: GGCGGTAGCCTCCTCCTTAG
RPL18	Ribosomal protein L18	MH196507	F: ATGGGATTTGGCTTCGTTATC	177	82.17	106.49	0.999
			R: AGAGTTTTGCTGGGATGGTG
HIS3	Histone H3.3	MH196508	F: GTTTCCAGAGCCACGCGG	156	84.93	92.83	0.999
			R: CACGCTCACCCCTGATCCTC
TUA3	Alpha-tubulin-3	MH196509	F: ATGGTATGATGCCCAGTGACACC	144	81.49	108.74	0.999
			R: GACGGTTATTGATGAAGTCAGGACTG
SAMDC	s-Adenosyl methionine decarboxylase	MH196510	F: CAGCTGAGTTCTCCATAGCCTTG	162	83.99	99.05	0.999
			R: AATCATCCTTGACAAAGCGGTG
TIP41	TIP41-like family protein	MH196511	F: TGATGGGGCACTAATGAGGC	202	81.07	126.43	0.996
			R: GTCTTATGCATGATCACAGGAAGC
UGPase	(UDP)-glucose pyrophosphorylase	MH196512	F: ACATTACAAGATGGCTTTGTTACCC	157	79.94	93.33	0.996
			R: CAGAAACCTTAAGGCTATCAAGCTC
18S	18S ribosomal RNA	AB636342.1	F: AAGTCGTAACAAGGTTTCCGTAG	80	80.10	118.84	0.999
			R: CCGATTCTCTGGTCGTTCTG
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	JQ731608.1	F: TACAGTTCCCGTGTGGTTGA	139	79.90	104.97	0.998
			R: CGAGAGGACGCAAGATAACA
PIP2	Plasma intrinsic protein 2	JX041626.1	F: ATCATCGGCACCTTCGTC	124	85.29	94.92	0.997
			R: GCACAATAAACACAGCAAACC
ACT	Actin	AB710173.1	F: CTTTCCCTCTATGCCAGTG	122	80.58	105.39	0.999
			R: CAAGGTCAAGCCTCAAGAT

ISSN: 1746-4811