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Table 1 Reference gene primer sequences and amplicon characteristics for each primer pair used in the RT-qPCR
| Gene | Gene description | GenBank ID | Primer sequence (5′–3′) | Amplicon length (bp) | Amplicon Tm (°C) | Amplification efficiency (%) | Regression coefficient (R2) |
|---|---|---|---|---|---|---|---|
| RPL4 | Ribosomal protein L4 | MH196506 | F: AGGTTCAGTCAGTCGTCAGGC | 215 | 81.59 | 102.91 | 0.994 |
| R: GGCGGTAGCCTCCTCCTTAG | |||||||
| RPL18 | Ribosomal protein L18 | MH196507 | F: ATGGGATTTGGCTTCGTTATC | 177 | 82.17 | 106.49 | 0.999 |
| R: AGAGTTTTGCTGGGATGGTG | |||||||
| HIS3 | Histone H3.3 | MH196508 | F: GTTTCCAGAGCCACGCGG | 156 | 84.93 | 92.83 | 0.999 |
| R: CACGCTCACCCCTGATCCTC | |||||||
| TUA3 | Alpha-tubulin-3 | MH196509 | F: ATGGTATGATGCCCAGTGACACC | 144 | 81.49 | 108.74 | 0.999 |
| R: GACGGTTATTGATGAAGTCAGGACTG | |||||||
| SAMDC | s-Adenosyl methionine decarboxylase | MH196510 | F: CAGCTGAGTTCTCCATAGCCTTG | 162 | 83.99 | 99.05 | 0.999 |
| R: AATCATCCTTGACAAAGCGGTG | |||||||
| TIP41 | TIP41-like family protein | MH196511 | F: TGATGGGGCACTAATGAGGC | 202 | 81.07 | 126.43 | 0.996 |
| R: GTCTTATGCATGATCACAGGAAGC | |||||||
| UGPase | (UDP)-glucose pyrophosphorylase | MH196512 | F: ACATTACAAGATGGCTTTGTTACCC | 157 | 79.94 | 93.33 | 0.996 |
| R: CAGAAACCTTAAGGCTATCAAGCTC | |||||||
| 18S | 18S ribosomal RNA | AB636342.1 | F: AAGTCGTAACAAGGTTTCCGTAG | 80 | 80.10 | 118.84 | 0.999 |
| R: CCGATTCTCTGGTCGTTCTG | |||||||
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | JQ731608.1 | F: TACAGTTCCCGTGTGGTTGA | 139 | 79.90 | 104.97 | 0.998 |
| R: CGAGAGGACGCAAGATAACA | |||||||
| PIP2 | Plasma intrinsic protein 2 | JX041626.1 | F: ATCATCGGCACCTTCGTC | 124 | 85.29 | 94.92 | 0.997 |
| R: GCACAATAAACACAGCAAACC | |||||||
| ACT | Actin | AB710173.1 | F: CTTTCCCTCTATGCCAGTG | 122 | 80.58 | 105.39 | 0.999 |
| R: CAAGGTCAAGCCTCAAGAT |
