Fig. 6

Hydrophobic cluster analysis and Trp-distribution in Lil3.2. The α-helical net duplicate of the amino acid sequences of LIL3:2 was analyzed and presented as hydrophobic cluster analysis (HCA) [25, 26]. Amino acids are plotted in one letter code and are colored or replaced by symbols [polar AA’s (N, D, E, Q), red; AA’s charged positively at neutral pH (K, R), blue; hydrophobic AA’s (F, I, V, L, W, M, Y), green]. Amino acids replaced by symbols are P, red star; T, empty square; S, square with smaller black square; G, black diamond, and W, filled red hexagon. Black outline highlights hydrophobic clusters and black lines under the plot were predicted as transmembrane regions by MPEx http://blanco.biomol.uci.edu/mpex/ (bottom) or as helical domains by i-Tasser (https://zhanglab.ccmb.med.umich.edu/I-TASSER/) (top). The HCA plot was subdivided according to the pattern of Prolin distribution in clusters (C) C1–C7. Key angular positions of amino acids relative to any amino acid (i) in an alpha-helical arrangement are provided in the direction of the protein sequence at + 100° (arrow, i + 1), − 60° (arrow, i + 3), and + 40° (arrow, i + 4) in the HCA plot