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Fig. 3 | Plant Methods

Fig. 3

From: A strategy to characterize chlorophyll protein interaction in LIL3

Fig. 3

DTT is oxidized at pH 11 and maintains solubility of LIL3. Absorbance spectra were recorded from 200 to 400 nm in a Shimadzu UV–VIS 2401PC spectrophotometer. DTT was dissolved in dH2O (DTT, pH 6, solid line) and reconstitution buffer (DTT, pH 11, broken line) (a). Spectra of LIL3 were recorded upon solubilization in DDM in reconstitution buffer at pH 11 in the absence (DDM) and presence of DTT (DDM, DTT) and upon reconstitution with Chl a (DDM, DTT, Chl a) (b)

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