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Fig. 4 | Plant Methods

Fig. 4

From: Combined use of cutinase and high-resolution mass-spectrometry to query the molecular architecture of cutin

Fig. 4

Chemical derivatization of non-esterified-hydroxyl- and carboxyl groups in intact cutin. a LC–MS/MS characterization of a cutinase hydrolysis product obtained from dansylated cutin; identification of the dansyl-derivative of 16-hydroxy-16:0 (m/z 506.30). b GC/MS characterization of a cutinase hydrolysis product obtained from benzyl-O-alkylated cutin; identification of 18-O-benzyl-derivative of 9,10,18-trihydroxy-18:0. c LC–MS/MS characterization of a cutinase hydrolysis product obtained from DmPA-derivatized cutin; identification of DmPA-derivatized 16-hydroxy-16:0 (m/z 434.38)

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