Table 1 Summary of genetic transformation of barley with different sgRNA constructs and PCR/RE screening of transformants for the presence of induced mutations

From: A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)

sgRNA construct	Edited gene	No. of explants	No. of transgenic events (%)	No. of independent transgenic plants	No. of PCR-RE detected mutants
ckx1-sgRNA	HvCKX1	1143	137 (12)	71	47
nud-sgRNA	Nud	1209	96 (8)	28	18
PTG-ckx1-ckx3	HvCKX1	1320	142 (11)	72	49	9^a
PTG-ckx1-ckx3	HvCKX3	1320	142 (11)	72	13	9^a

^aNo. of plants with mutations in both the HvCKX1 and HvCKX3 genes

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ISSN: 1746-4811

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