Fig. 4

Phenotype changes generated by knock-out mutations of the Nud gene in barley. a Mature spikes of wild-type control (left) and nud mutant (right) plants; yellow arrowheads indicate opened hulls surrounding a naked caryopsis (all grains in the spike are naked but the hull openings are visible only at a specific angle). b Examples of mature covered grains from a wild-type plant (upper row) and naked grains from a nud mutant plant (bottom row); c sequence alignment of the Nud gene fragments cloned from T₀ biallelic mutants. Target sequence is marked in yellow and PAM motif in light blue; deletions are indicated by dashes, and insertions by red letters