Fig. 1

Overview of methods. a Scheme depicting the ATAC-seq method. Transposase (pentagons) digests the chromatin more often in accessible sites and deposits its adapters (orange and purple), which are used for library preparation and sequencing. b Illustration of the INTACT construct. p35S, 35S promoter; WPP, nuclear envelope targeting domain; GFP, green fluorescent protein; BT, biotinylatable tag. c Nuclei isolated using the INTACT method. The nuclei are stained with DAPI (blue) and are bound by streptavidin-coated beads (green). Scale bar: 10 µm