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Table 1 Overview of the main soluble mucilage- and seed shape-releated parameters computed by MuSeeQ

From: MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parameters

Traits MuSeeQ designation Description Code
Seed Area (mm2) The area of the seed surface projected in 2D. Computed as: AreaS = π × radius2 AreaS
Circularity Corresponds to a more or less elongated seed shape. Circularity is comprised between 0.0 and 1.0. A value of 1.0 indicates a perfectly round seed. Computed as: CircS = 4π(AreaS/PeriS2) CircS
Feret (mm) The longest distance between two points of the segmented seed. For seeds, it can be interpreted as the longest distance between the pedoncule and the awn FerS
Perimeter (mm) The length of the limit of the segmented seed. Computed as: PeriS = 2 × π × radius PeriS
Solidity Corresponds to the ratio between the exact and the approximated seed area, using a sort of rubber band all around the segmented seed. Computed as: SolS = AreaS/Convex AreaS SolS
Soluble Mucilage Area (mm2) The area of the surface of released soluble mucilage projected in 2D. Computed as: AreaM = π × radius2 AreaM
Circularity Corresponds to a more or less elongated mucilage shape. Circularity is comprised between 0.0 and 1.0. A value of 1.0 indicates a perfectly round mucilage. Computed as: CircM = 4π(AreaM/PeriM2) CircM
Perimeter (mm) The length of the limit of the segmented mucilage. Computed as: PeriM = 2 × π × radius PeriM
Solidity Corresponds to the ratio between the exact and approximated mucilage areas using a sort of rubber band all around the segmented mucilage. Computed as: SolM = AreaM/Convex AreaM SolM
Ajusted mucilage content The 2D surface of released mucilage corrected by its corresponding seed surface projected in 2D Ratio (AreaM/AreaS)
  1. Please note that the number of parameters which could be analysed can be increased using other plugins and functions in ImageJ