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Table 1 Overview of the main soluble mucilage- and seed shape-releated parameters computed by MuSeeQ

From: MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parameters

Traits

MuSeeQ designation

Description

Code

Seed

Area (mm2)

The area of the seed surface projected in 2D. Computed as: AreaS = π × radius2

AreaS

Circularity

Corresponds to a more or less elongated seed shape. Circularity is comprised between 0.0 and 1.0. A value of 1.0 indicates a perfectly round seed. Computed as: CircS = 4π(AreaS/PeriS2)

CircS

Feret (mm)

The longest distance between two points of the segmented seed. For seeds, it can be interpreted as the longest distance between the pedoncule and the awn

FerS

Perimeter (mm)

The length of the limit of the segmented seed. Computed as: PeriS = 2 × π × radius

PeriS

Solidity

Corresponds to the ratio between the exact and the approximated seed area, using a sort of rubber band all around the segmented seed. Computed as: SolS = AreaS/Convex AreaS

SolS

Soluble Mucilage

Area (mm2)

The area of the surface of released soluble mucilage projected in 2D. Computed as: AreaM = π × radius2

AreaM

Circularity

Corresponds to a more or less elongated mucilage shape. Circularity is comprised between 0.0 and 1.0. A value of 1.0 indicates a perfectly round mucilage. Computed as: CircM = 4π(AreaM/PeriM2)

CircM

Perimeter (mm)

The length of the limit of the segmented mucilage. Computed as: PeriM = 2 × π × radius

PeriM

Solidity

Corresponds to the ratio between the exact and approximated mucilage areas using a sort of rubber band all around the segmented mucilage. Computed as: SolM = AreaM/Convex AreaM

SolM

Ajusted mucilage content

The 2D surface of released mucilage corrected by its corresponding seed surface projected in 2D

Ratio (AreaM/AreaS)

  1. Please note that the number of parameters which could be analysed can be increased using other plugins and functions in ImageJ