Fig. 5From: MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parametersMuSeeQ allows to quantitatively discriminate between close soluble mucilage phenotypes. Pictures showing flaxseeds sown on (a) a biochemical assay directly or (b) after 24 h of soluble mucilage extraction in water. In both cases, pictures have been taken after 24 h of mucilage release on the gels. White arrows indicate that the seeds are capable of releasing the adherent part of the mucilage on the gel (b). c Soluble mucilage phenotyping in water containing toluidine blue O after 24 h of release. d Soluble mucilage phenotyping using the MuSeeQ’s biochemical assay after 24 h of release. Seeds in (c) and (d) were chosen as the most representative of the corresponding RILs. e Statistical analysis of the ratio (AreaM/AreaS) showing different phenotypes among the three RILs. Significant differences were determined using a one-way ANOVA followed by a Tukey’s HSD post hoc test, *p < 0.05; ***p < 0.001 (n > 21 seeds for each RIL). Bars: 2 mmBack to article page