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Fig. 5 | Plant Methods

Fig. 5

From: MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parameters

Fig. 5

MuSeeQ allows to quantitatively discriminate between close soluble mucilage phenotypes. Pictures showing flaxseeds sown on (a) a biochemical assay directly or (b) after 24 h of soluble mucilage extraction in water. In both cases, pictures have been taken after 24 h of mucilage release on the gels. White arrows indicate that the seeds are capable of releasing the adherent part of the mucilage on the gel (b). c Soluble mucilage phenotyping in water containing toluidine blue O after 24 h of release. d Soluble mucilage phenotyping using the MuSeeQ’s biochemical assay after 24 h of release. Seeds in (c) and (d) were chosen as the most representative of the corresponding RILs. e Statistical analysis of the ratio (AreaM/AreaS) showing different phenotypes among the three RILs. Significant differences were determined using a one-way ANOVA followed by a Tukey’s HSD post hoc test, *p < 0.05; ***p < 0.001 (n > 21 seeds for each RIL). Bars: 2 mm

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