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Fig. 3 | Plant Methods

Fig. 3

From: MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphometric parameters

Fig. 3

The work flow of the labelling and numbering pipeline. a Raw image from the biochemical assay. b Initial binary image from the first raw image duplicata after segmentation of the soluble mucilage halos around the seeds (purple-pink). c Real area of the soluble mucilages segmented by MuSeeQ including pixels below the seeds. d Final step of the segmentation of the soluble mucilages after labelling and numbering. e Initial binary image from the second raw image duplicata after segmentation of the seeds. f Final step of the segmentation of the seeds after labelling and numbering. g–i High-magnification views of one seed and its corresponding soluble mucilage both numbered 28 and stained in light green. Bars: 3 mm

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