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Fig. 4 | Plant Methods

Fig. 4

From: User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae

Fig. 4

Multistage tandem mass spectrometry analysis allowing the determination of the structure of the permethylated LLO-released oligosaccharide isolated from the diatom P. tricornutum. ESI-MSn spectra with n = 2 selecting m/z 1413.5 ([M + 2Na]2+) as the precursor ion (a), n = 3 selecting m/z 1492.5 ([M + Na]+) as intermediate ion (b) and n = 3 with m/z 1682.5 ([M + Na]+) as intermediate ion (c) of permethylated Hex11HexNAc2 derivative) isolated from P. tricornutum. On each panel, the ion selected for the fragmentation analysis is shown with a diamond and its fragmentation pattern is proposed according to Prien et al. [40]. Black square: N-acetylglucosamine; grey circle: mannose, black circle: glucose. The fragment ions are labelled according to the nomenclature of Domon and Costello [41]

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