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Table 1 ABA content in different tissues of Arabidopsis and rice (ng/g, fresh weight)

From: Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR

Plant tissue

qIPCR

HPLC–MS/MS

Purified sample

Crude extract

Purified sample

Crude extract

Arabidopsis root

45.1 ± 1.5

46.1 ± 2.3

43.3 ± 0.7

–

Arabidopsis stem

41.2 ± 1.1

39.2 ± 1.7

42.4 ± 0.8

–

Arabidopsis leaf

35.4 ± 0.8

36.4 ± 2.1

34.8 ± 0.5

–

Arabidopsis flower

55.6 ± 1.3

58.6 ± 2.8

58.2 ± 0.9

–

Arabidopsis seed

289.2 ± 1.9

293.9 ± 4.3

276.5 ± 1.8

–

Rice seedling root

47.3 ± 0.8

46.3 ± 2.6

48.7 ± 1.1

–

Rice seedling leaf

32.9 ± 1.3

32.7 ± 1.8

33.5 ± 0.9

–

Mature rice seed

313.6 ± 3.1

317.4 ± 3.9

305 ± 2.2

–

  1. The purified sample was collected through standard extraction and purification processes. The crude extract was dissolved in acetonitrile after extracting with 80% acetone, removing solid impurity and drying [36]. “–” Represented no data because crude extract was not allowed in HPLC–MS/MS. Data represent the means and SD of three independent biological replicates (n = 5)
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Plant Methods

ISSN: 1746-4811