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Table 1 ABA content in different tissues of Arabidopsis and rice (ng/g, fresh weight)

From: Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR

Plant tissue qIPCR HPLC–MS/MS
Purified sample Crude extract Purified sample Crude extract
Arabidopsis root 45.1 ± 1.5 46.1 ± 2.3 43.3 ± 0.7
Arabidopsis stem 41.2 ± 1.1 39.2 ± 1.7 42.4 ± 0.8
Arabidopsis leaf 35.4 ± 0.8 36.4 ± 2.1 34.8 ± 0.5
Arabidopsis flower 55.6 ± 1.3 58.6 ± 2.8 58.2 ± 0.9
Arabidopsis seed 289.2 ± 1.9 293.9 ± 4.3 276.5 ± 1.8
Rice seedling root 47.3 ± 0.8 46.3 ± 2.6 48.7 ± 1.1
Rice seedling leaf 32.9 ± 1.3 32.7 ± 1.8 33.5 ± 0.9
Mature rice seed 313.6 ± 3.1 317.4 ± 3.9 305 ± 2.2
  1. The purified sample was collected through standard extraction and purification processes. The crude extract was dissolved in acetonitrile after extracting with 80% acetone, removing solid impurity and drying [36]. “–” Represented no data because crude extract was not allowed in HPLC–MS/MS. Data represent the means and SD of three independent biological replicates (n = 5)