Fig. 4

Binding saturation analysis between ABA and ABA McAb. ABA McAb (400 ng) was coated on inner PCR tube, then 2 ng ABA in 20 µL PBS was added into PCR tube and the mixture was incubated at 4 °C in dark. The solution in PCR tube was removed at half hour interval and ABA concentration was determined by LC–MS/MS. Data represent the means and SD of three independent biological replicates (n = 8)