Histology-guided high-resolution AP-SMALDI mass spectrometry imaging of wheat- Fusarium graminearum interaction at the root–shoot junction

Table 1 Selected metabolites assigned in wheat stem tissues by AP-SMALDI-MS imaging

Compounds	Location in stem base			LMI^a	References^b
Compounds	10 dai	14 dai	21 dai	LMI^a	References^b
Host-derived
Feruloylagmatine	Leaf sheath (ep, vb)		Leaf sheath (vb)	1	[31, 32]
Iridotrial glucoside	Leaf sheath (ep, vb)			2	[31, 33]
Oxo-octadecadiynoic acid	Leaf sheath (ep, vb)			1	[38]
Adenosyl methionine	Leaf sheath (ep)			1	[31]
Kaempferol methyl ether glucuronide		Stem		2
Corchorusoside E		Stem (vb)		1
Coumaroylagmatine			Leaf sheath (vb), stem (co)	1	[31,32,33]
Feruloylserotonin			Leaf sheath (vb)	1	[31, 32]
Coumaroylserotonin			Leaf sheath (vb)	1	[31,32,33]
PA(38:3)		Leaf sheath	Leaf sheath	2
PS(36:3)		Leaf sheath	Leaf sheath	2
Pathogen-derived
Cerebroside C	Leaf sheath (ep)
Enniatin B		Leaf sheath (ep)	Leaf sheath	1
Enniatin B2			Leaf sheath	1
Enniatin B4			Leaf sheath	1
Enniatin A1			Leaf sheath	1
Beauvericin			Leaf sheath	1

Additional file 2: Table S1 provides a complete list of annotated compounds, locations, molecular formulas, adducts, and theoretical masses. Abbreviations: co, specific location in cortex parenchyma; dai, days after root inoculation with F. graminearum; ep, specific location in epidermis; PA, phosphatidic acid; PS, phosphatidylserine; vb, specific location in vascular bundles
^aLevel of metabolite identification (LMI) in public databases: 1, putative compound (match to a single compound); 2, putatively characterized compound class or representative of a compound class (match to isomeric metabolites)
^bReferences (Ref) for metabolites detected in studies on wheat or barely resistance to the spike disease Fusarium head blight caused by F. graminearum