Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.)

Table 5 Effect of different combinations of enzymes on protoplast isolation

Enzyme combination		Total no. of protoplasts (× 10⁵)	Total no. of living protoplasts (× 10⁵)	Viability %
Macerozyme R-10%	Cellulase R-10%	Total no. of protoplasts (× 10⁵)	Total no. of living protoplasts (× 10⁵)	Viability %
0.25	0.5	1.7 ± 0.2^d	0.8 ± 0.05^DE	50
0.50	0.5	1.6 ± 1.1^d	0.6 ± 0.25^DE	41
0.75	0.5	2.4 ± 0.6^d	0.5 ± 0.30^E	24
0.25	1.0	5.6 ± 0.5^ab	2.1 ± 0.30^BC	31
0.50	1.0	2.5 ± 0.1^d	1.0 ± 0.33^DE	41
0.75	1.0	2.2 ± 0.6^d	0.7 ± 0.25^DE	33
0.25	1.5	5.0 ± 2.8^abc	2.5 ± 1.50^AB	50
0.50	1.5	6.5 ± 1.4^a	3.3 ± 0.60^A	51
0.75	1.5	1.8 ± 1.3^c	0.8 ± 0.57^DE	44
0.25	2.0	4.6 ± 0.4^bc	2.3 ± 0.26^BC	50
0.50	2.0	4.8 ± 0.5^abc	1.5 ± 0.17^CD	32
0.75	2.0	3.1 ± 0.1^cd	4.6 ± 0.16^E	15

Grouping information based on Fisher’s method. Means that do not share the same letter are significantly different, with a 95% confidence interval

ISSN: 1746-4811