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Table 5 Effect of different combinations of enzymes on protoplast isolation

From: Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.)

Enzyme combination Total no. of protoplasts (× 105) Total no. of living protoplasts (× 105) Viability %
Macerozyme R-10% Cellulase R-10%
0.25 0.5 1.7 ± 0.2d 0.8 ± 0.05DE 50
0.50 0.5 1.6 ± 1.1d 0.6 ± 0.25DE 41
0.75 0.5 2.4 ± 0.6d 0.5 ± 0.30E 24
0.25 1.0 5.6 ± 0.5ab 2.1 ± 0.30BC 31
0.50 1.0 2.5 ± 0.1d 1.0 ± 0.33DE 41
0.75 1.0 2.2 ± 0.6d 0.7 ± 0.25DE 33
0.25 1.5 5.0 ± 2.8abc 2.5 ± 1.50AB 50
0.50 1.5 6.5 ± 1.4a 3.3 ± 0.60A 51
0.75 1.5 1.8 ± 1.3c 0.8 ± 0.57DE 44
0.25 2.0 4.6 ± 0.4bc 2.3 ± 0.26BC 50
0.50 2.0 4.8 ± 0.5abc 1.5 ± 0.17CD 32
0.75 2.0 3.1 ± 0.1cd 4.6 ± 0.16E 15
  1. Grouping information based on Fisher’s method. Means that do not share the same letter are significantly different, with a 95% confidence interval