Enzyme combination | Total no. of protoplasts (× 105) | Total no. of living protoplasts (× 105) | Viability % | |
---|---|---|---|---|
Macerozyme R-10% | Cellulase R-10% | |||
0.25 | 0.5 | 1.7 ± 0.2d | 0.8 ± 0.05DE | 50 |
0.50 | 0.5 | 1.6 ± 1.1d | 0.6 ± 0.25DE | 41 |
0.75 | 0.5 | 2.4 ± 0.6d | 0.5 ± 0.30E | 24 |
0.25 | 1.0 | 5.6 ± 0.5ab | 2.1 ± 0.30BC | 31 |
0.50 | 1.0 | 2.5 ± 0.1d | 1.0 ± 0.33DE | 41 |
0.75 | 1.0 | 2.2 ± 0.6d | 0.7 ± 0.25DE | 33 |
0.25 | 1.5 | 5.0 ± 2.8abc | 2.5 ± 1.50AB | 50 |
0.50 | 1.5 | 6.5 ± 1.4a | 3.3 ± 0.60A | 51 |
0.75 | 1.5 | 1.8 ± 1.3c | 0.8 ± 0.57DE | 44 |
0.25 | 2.0 | 4.6 ± 0.4bc | 2.3 ± 0.26BC | 50 |
0.50 | 2.0 | 4.8 ± 0.5abc | 1.5 ± 0.17CD | 32 |
0.75 | 2.0 | 3.1 ± 0.1cd | 4.6 ± 0.16E | 15 |