Fig. 2From: Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.)Proliferation of callus and in vitro plant growth. a Fragile embryonic callus. b Hard highly compact non-embryonic callus. c Small plantlets start to grow from embryonic callus. d Well-grown callus with small plantlets after 1 month (e). Plants grown in MS liquid medium for 1 month (f). Acclimatized in vitro grown plants. Bar in a–c = 1000 μmBack to article page