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Fig. 4 | Plant Methods

Fig. 4

From: Transient co-expression with three O-glycosylation enzymes allows production of GalNAc-O-glycosylated Granulocyte-Colony Stimulating Factor in N. benthamiana

Fig. 4

a, b Golgi and apoplast localization of Sec-G-CSF in N. benthamiana plants. Sec-G-CSF:eYFP (A, C, D), SecGFP (E, G, H), GFP:KDEL (I, K, L). Sec-G-CSF, SecGFP and GFP:KDEL were co-infiltrated with STM:mRFP (B, C, F, G, J, K). White arrows depict Golgi localization (A, B, E, F) and colocalization (C, G) with STM:mRFP. White arrowheads (images on the right) depict localization in apoplast (D, H), or apoplast without presence of recombinant protein (I). Agrobacterium AGL-1 was carrying Sec-G-CSF. SecGFP, GFP:KDEL, STM:mRFP and p19 were delivered by EHA105. 4 dpi. Size bar: 5 μm. a shows the colors as originally displayed, while b shows a color palette adjusted for those who have difficulty viewing red and green colors

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Plant Methods

ISSN: 1746-4811