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Fig. 1 | Plant Methods

Fig. 1

From: Transient co-expression with three O-glycosylation enzymes allows production of GalNAc-O-glycosylated Granulocyte-Colony Stimulating Factor in N. benthamiana

Fig. 1

Schematic representation of constructs generated in this work and used for Agrobacterium-mediated transient expression in N. benthamiana leaves. Sec-G-CSF:eYFP, secretory G-CSF variant fused to C-terminal eYFP; Cyt-G-CSF:eYFP, cytoplasmic G-CSF variant fused to C-terminal eYFP; Zera-G-CSF:eYFP, G-CSF fused to N-terminal Zera peptide and C-terminal eYFP; Sec-G-CSF:cMyc, secretory G-CSF fused to C-terminal c-Myc4x. 35S-P, Cauliflower Mosaic Virus 35S promoter; nos-T, nopaline synthase transcription terminator; G-CSF, human Granulocyte-Colony Stimulating Factor coding sequence; Nat-SP, native secretory signal peptide from human G-CSF; Zera, γ-Zein ER-accumulating domain; Ek, enterokinase cleavage site; eYFP, enhanced yellow fluorescent protein for confocal and serological detection; c-Myc4x, four repeats of the c-Myc tag for detection and purification; attB1 and attB2, linker regions derived from recombination sites generated after Gateway cloning. Schematic representation not drawn to scale

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Plant Methods

ISSN: 1746-4811