Fig. 2
From: Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH)
Probe validation using clone-FISH and evaluation of cross-reactivity with non-target oomycetes. a–c Positive control: Escherichia coli DE3 cells expressing the Plasmopara obducens target sequence; d–f negative control: E. coli DE3 cells carrying a self-ligated plasmid without the P. obducens target sequence insert; g–i negative controls: Phytophthora infestans mycelia and spore; j–l Phy. sojae mycelia; m–o, Pythium irregulare mycelia and spores. Scale bar: a–c, g–o = 20 µm; d–f = 10 µm