Table 1 Percentage of blue and white E. coli colonies resulting from various Pyrite cloning applications shown in Fig. 2
From: Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes
Application type | Figure 2 | Blue colonies | Total colonies | Percent blue | Percent white |
|---|---|---|---|---|---|
Background control | C | > 100 | > 100 | 100 | 0 |
Insertion | D | 0 | > 100 | 0 | 100 |
Use of BamHI | E | 78 | 458 | 17.0 | 83.0 |
Simultaneous cloning (pUC19) | F | 0 | 202 | 0 | 100 |
Simultaneous cloning (pSanFran) | n/a | n/a | 401 | n/a | 100 |
Vector swap | G | 319 | 629 | 50.7 | 49.3 |