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Table 3 Flag leaf stomatal conductance (gs, mol m−2 s−1), internal [CO2] (Ci, µmol CO2 mol−1 air), total soluble proteins (TSP, g m−2), total Rubisco activity (Vt, µmol CO2 m−2 s−1) and Rubisco activation state (Act%, %) before and after foliar application of ABA to the leaves of the wild-type (WT) barley genotype Steptoe and the ABA-deficient mutant Az34

From: Whole plant chamber to examine sensitivity of cereal gas exchange to changes in evaporative demand

  Leaf gs Leaf Ci Leaf TSP Leaf Rubisco Vt Leaf Rubisco Act %
WT 0.26 ± 0.01 292 ± 5 7.44 ± 1.56 46.9 ± 12.1 65.9 ± 7.5
WT + ABA 0.14 ± 0.03 245 ± 29 8.10 ± 1.54 43.7 ± 10.4 66.4 ± 3.2
% change after ABA treatment − 47 − 16 N.s. N.s. N.s.
Az34 0.39 ± 0.05 332 ± 9 3.57 ± 0.39 17.0 ± 1.4 76.0 ± 2.9
Az34 + ABA 0.08 ± 0.02 234 ± 14 3.81 ± 0.4 19.8 ± 2.8 56.3 ± 3.8
% change after ABA treatment − 79 − 30 N.s. N.s. − 26
Genotype 0.017 0.081 < 0.001 < 0.001 0.601
ABA treatment < 0.001 < 0.001 0.839 0.960 < 0.001
Genotype * ABA treatment < 0.001 0.009 0.200 0.450 < 0.001
  1. The percentage change in response to ABA treatments is calculated when significant. Data are mean ± SE of 4 replicates. Results from two-way ANOVA (P values) are presented for Genotype, ABA treatment and their interaction
  2. N.s. not significant