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Fig. 2 | Plant Methods

Fig. 2

From: Cryo-laser scanning confocal microscopy of diffusible plant compounds

Fig. 2

Effect of cryo-fixation methods on the apple cortex and grape berry tissue structure: ab frozen samples at − 20 °C; cd fast-frozen samples in liquid nitrogen; ef fast-frozen samples in cooled isopentane; and gh frozen samples at − 30 °C under microwaves. All sections were prepared from apples (a, c, e, g) or grapes (b, d, f, h) and stained by infusion with Acridine Orange. CW: Cell wall; C: Cuticle. Scale bar: 100 and 10 µm in inset

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