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Table 6 qPCR descriptive statistics of the candidate reference genes in cowpea under root dehydration and salt stress (NaCl, 100 mM), measured by BestKeeper software

From: Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR

Abiotic stress Candidate reference gene Rank Pairwise correlation (PC) coefficient Min Max SD PC coefficient p value
GM AM min max SD CV [x-fold] [x-fold] [± x-fold] [r]
Salt stress UNK 1 19.91 19.93 18.60 22.10 0.64 3.24 − 2.47 4.50 1.58 0.982 0.001
β-TUB 2 19.81 19.85 18.00 23.40 1.00 5.03 − 3.60 12.71 2.03 0.979 0.001
FBOX 3 21.59 21.61 20.10 24.10 0.68 3.13 − 2.85 5.84 1.62 0.975 0.001
UE21D 4 18.40 18.42 17.00 20.70 0.60 3.24 − 2.65 4.99 1.52 0.975 0.001
VuUBQ10 5 15.55 15.58 13.80 18.00 0.78 5.03 − 3.27 5.28 1.74 0.920 0.001
VuACT 6 20.41 20.43 18.70 23.00 0.66 3.23 − 3.44 6.50 1.59 0.915 0.001
EF1-α* 7 16.58 16.68 14.40 22.80 1.36 8.16 − 4.43 70.08 2.62 0.948 0.001
Root dehydration β-TUB 1 19.93 19.95 18.60 21.30 0.55 2.76 − 2.57 2.63 1.48 0.958 0.001
UNK 2 20.35 20.37 19.30 22.20 0.60 2.95 − 2.07 3.56 1.53 0.925 0.001
VuACT 3 21.74 21.75 20.60 23.50 0.53 2.45 − 2.28 3.56 1.46 0.910 0.001
UE21D 4 19.04 19.05 18.07 20.40 0.51 2.65 − 1.97 2.59 1.43 0.875 0.001
FBOX 5 22.16 22.17 21.20 23.70 0.56 2.52 − 1.96 2.96 1.48 0.870 0.001
VuUBQ10 6 16.57 16.58 15.80 17.90 0.45 2.70 − 1.68 2.47 1.37 0.816 0.001
EF1-α 7 16.84 16.85 15.80 18.50 0.47 2.78 − 2.03 3.11 1.39 0.475 0.019
  1. Vu (Vigna unguiculata); UNK (Phaseolus vulgaris unknown gene); β-TUB (beta-tubulin); FBOX (F-box protein); UE21D (ubiquitin-conjugating enzyme E2 variant 1D); VuUBQ10 (polyubiquitin 10); VuACT (actin); EF1-α (elongation Factor 1-alfa); GM: the geometric mean of PC; AM: the arithmetic mean of PC; Min PC and Max PC: the extreme values of PC; SD: the standard deviation of the PC; CV: the coefficient of variance expressed as a percentage of the PC level; Min [x-fold] and Max [x-fold]: the extreme values of expression levels expressed as an absolute x-fold over- or under-regulation coefficient; SD [± x-fold]: standard deviation of the absolute regulation coefficient; [r]: Pearson correlation coefficient
  2. *Inconsistent data: SD > 1 [11]