Table 6 qPCR descriptive statistics of the candidate reference genes in cowpea under root dehydration and salt stress (NaCl, 100 mM), measured by BestKeeper software
From: Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR
Abiotic stress | Candidate reference gene | Rank | Pairwise correlation (PC) coefficient | Min | Max | SD | PC coefficient | p value | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GM | AM | min | max | SD | CV | [x-fold] | [x-fold] | [± x-fold] | [r] | ||||
Salt stress | UNK | 1 | 19.91 | 19.93 | 18.60 | 22.10 | 0.64 | 3.24 | − 2.47 | 4.50 | 1.58 | 0.982 | 0.001 |
β-TUB | 2 | 19.81 | 19.85 | 18.00 | 23.40 | 1.00 | 5.03 | − 3.60 | 12.71 | 2.03 | 0.979 | 0.001 | |
FBOX | 3 | 21.59 | 21.61 | 20.10 | 24.10 | 0.68 | 3.13 | − 2.85 | 5.84 | 1.62 | 0.975 | 0.001 | |
UE21D | 4 | 18.40 | 18.42 | 17.00 | 20.70 | 0.60 | 3.24 | − 2.65 | 4.99 | 1.52 | 0.975 | 0.001 | |
VuUBQ10 | 5 | 15.55 | 15.58 | 13.80 | 18.00 | 0.78 | 5.03 | − 3.27 | 5.28 | 1.74 | 0.920 | 0.001 | |
VuACT | 6 | 20.41 | 20.43 | 18.70 | 23.00 | 0.66 | 3.23 | − 3.44 | 6.50 | 1.59 | 0.915 | 0.001 | |
EF1-α* | 7 | 16.58 | 16.68 | 14.40 | 22.80 | 1.36 | 8.16 | − 4.43 | 70.08 | 2.62 | 0.948 | 0.001 | |
Root dehydration | β-TUB | 1 | 19.93 | 19.95 | 18.60 | 21.30 | 0.55 | 2.76 | − 2.57 | 2.63 | 1.48 | 0.958 | 0.001 |
UNK | 2 | 20.35 | 20.37 | 19.30 | 22.20 | 0.60 | 2.95 | − 2.07 | 3.56 | 1.53 | 0.925 | 0.001 | |
VuACT | 3 | 21.74 | 21.75 | 20.60 | 23.50 | 0.53 | 2.45 | − 2.28 | 3.56 | 1.46 | 0.910 | 0.001 | |
UE21D | 4 | 19.04 | 19.05 | 18.07 | 20.40 | 0.51 | 2.65 | − 1.97 | 2.59 | 1.43 | 0.875 | 0.001 | |
FBOX | 5 | 22.16 | 22.17 | 21.20 | 23.70 | 0.56 | 2.52 | − 1.96 | 2.96 | 1.48 | 0.870 | 0.001 | |
VuUBQ10 | 6 | 16.57 | 16.58 | 15.80 | 17.90 | 0.45 | 2.70 | − 1.68 | 2.47 | 1.37 | 0.816 | 0.001 | |
EF1-α | 7 | 16.84 | 16.85 | 15.80 | 18.50 | 0.47 | 2.78 | − 2.03 | 3.11 | 1.39 | 0.475 | 0.019 | |