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Fig. 4 | Plant Methods

Fig. 4

From: Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Fig. 4

Detection of CRISPR/Cas9-mediated mutation efficiency using the frame-shift GUS (fsGUS) system. a Diagram of the fsGUS reporting system. The 23 bp target sequence of sgRNA-PDS was inserted after the GUS start codon to generate fsGUS (p35S::fsGUS). CRISPR/Cas9 constructs (pAtU6::sgRNA and pGhU6::sgRNA) were co-expressed with p35S::fsGUS in cotton cotyledons, the p35S::GUS was used as positive control. b GUS staining of cotton cotyledons co-transfected with fsGUS and CRISPR/Cas9 constructs. c GUS activity assays of cotton cotyledons co-transfected with fsGUS and CRISPR/Cas9 constructs (n > 8, **P < 0.01, t-test)

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