Fig. 1
From: Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
Identification and validation of cotton U6 promoters. a Multiple alignments of cotton and Arabidopsis U6 gene and promoter sequences. Black line denotes the U6 snRNA transcript. USE (upstream sequence element), TATA-like Box and the transcription start site are labeled with red boxes. b Schematic representation of GUS expression constructs using different GhU6.3 promoter fragments. c GUS activity levels and d GUS staining in tobacco leaves infiltrated with Agrobacterium carrying different promoter constructs. The error bars indicate the standard deviation estimated from the eight replicates