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Fig. 3 | Plant Methods

Fig. 3

From: Functional intron-derived miRNAs and host-gene expression in plants

Fig. 3

Dual activity of ffgLUCaimiR-319a in transient assays (a) and stable transformed plants (b, c). a Left: LUC activity of ffgLUC and ffgLUCaimiR-319a at 4 days post-agro-infiltration in Nicotiana benthamiana transient assay (n = five leaves per treatment). Right: RT-PCR analysis of NbTCP4 expression in transient assay with ffgLUC or ffgLUC aimiR-319adel . Quantification of the DsRED gene expression was used to confirm similar transformation efficiencies in the agro-infiltration with ffgLUC and ffgLUCaimiR-319a (Additional file 1: Fig. S3). b Left: Representative stable transformed Arabidopsis thaliana rosette plant and leaf expressing ff-gLUC or ffgLUCaimiR-319a Right: average relative expression level of TCP2/3/4 genes in five WT and ffgLUC aimiR-319adel plants (reference gene AthActin1, expression of TCP2,3 and 4 each normalized to that in one WT plant). Significant differences between samples (***) is based on standard error (student’s t test, P < 0.01). c Left: LUC activity in representative stable transformant Arabidopsis thaliana expressing ff-gLUC or ffgLUCaimiR-319a. Right: quantified LUC in expression of eight independent transformants expressing either ff-gLUC or ffgLUCaimiR-319a

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