Fig. 2
From: Functional intron-derived miRNAs and host-gene expression in plants
Single activity of ffgLUC aimiR-319adel in transient assays and stable transformed plants. a Left: LUC activity of ffgLUC and ffgLUC aimiR-319adel at 4 days post-agro-infiltration in Nicotiana benthamiana transient assay. Significant differences between samples (***) is based on standard error (student’s t test, P < 0.01). Right: RT-PCR analysis of NbTCP4 expression in transient assay with ffgLUC or ffgLUC aimiR-319adel . Quantification of the DsRED gene expression was used to confirm similar transformation efficiencies in the agro-infiltration with ffgLUC and ffgLUC aimiR-319adel (Additional file 1: Fig. S3). b Left: Representative stable transformed Arabidopsis rosette plant and leaf expressing ff-gLUC or ffgLUC aimiR-319adel . Right: (reference gene AthActin1, expression of TCP 2, 3 and 4 each normalized to that in one WT plant). Significant differences between samples (***) is based on standard error (student’s t test, P < 0.01). c LUC activity in representative stable transformant Arabidopsis expressing ff-gLUC or ffgLUC aimiR-319adel . Graph: quantified LUC expression of eight independent transformants expressing either ff-gLUC or ffgLUC aimiR-319adel . d Top: PCR forward and reverse primer positions in ffgLUC aimiR-319adel . Bottom: PCR products on RNA isolated from ffgLUC aimiR-319adel plants. At each band position the structure of the mRNA sequence is shown (see also Additional file 1: Fig. S2)