Treatment, plant growth regulators (mg/L) | Explant forming callus (%) | Type of callus | Explant forming embryos (%) | Number of embryos | ||||||
---|---|---|---|---|---|---|---|---|---|---|
TDZ | BAP | 2,4-D | Light | Dark | Light | Dark | Light | Dark | Light | Dark |
0.1 | 16.7 ± 10cd | 0d | IV (+) | – | 0f | 0f | 0e | 0e | ||
0.5 | 10 ± 10 cd | 0d | IV (+) | – | 0f | 0f | 0e | 0e | ||
1 | 43.3 ± 10bc | 0d | IV (+) | – | 0f | 0f | 0e | 0e | ||
0.1 | 0.1 | 96,7 ± 3.3a | 96.7 ± 3.3a | I, II (+++) | I, III (++) | 56.7 ± 3.3a–d | 83.3 ± 3.3a | 17.5 ± 5.5a–d | 31.0 ± 5.0a | |
0.5 | 0.1 | 100a | 100a | I, II (+++) | I, III(+++) | 43.3 ± 10b–e | 66.7 ± 6.7a–c | 8.0 ± 3.0c–e | 17.5 ± 3.5a–d | |
1 | 0.1 | 96.7 ± 3.3a | 100a | I, II (++) | I, III (+++) | 26.7d–f | 60.0 ± 13.3a–c | 4.5 ± 0.5de | 11.5 ± 2.5b–e | |
0.1 | 0d | 0d | – | – | 0f | 0f | 0e | 0e | ||
0.5 | 0d | 0d | – | – | 0f | 0f | 0e | 0e | ||
1 | 0d | 0d | – | – | 0f | 0f | 0e | 0e | ||
0.1 | 0.1 | 73.3 ± 13.3ab | 83.3 ± 10ab | I, II (+) | I, III (+) | 40.0c–e | 76.7 ± 3.3a | 12.5 ± 3.5b−e | 28.5 ± 5.5a | |
0.5 | 0.1 | 93.3 ± 6.7a | 96.7 ± 3.3a | I, II (++) | I, III (++) | 13.30ef | 73.3ab | 4.5 ± 1.5de | 20.0 ± 1.0a−c | |
1 | 0.1 | 63.3 ± 23.3ab | 86.7 ± 6.7a | I, II (++) | I, III (++) | 16.7 ± 16.7ef | 80.0 ± 6.7a | 4.0 ± 4.0de | 24.5 ± 2.5ab | |
0.1 | 0d | 0d | – | – | 0f | 0f | 0e | 0e |